NEW SOLUBLE-FORMAZAN ASSAY FOR HIV-1 CYTOPATHIC EFFECTS - APPLICATION TO HIGH-FLUX SCREENING OF SYNTHETIC AND NATURAL-PRODUCTS FOR AIDS-ANTIVIRAL ACTIVITY
NEW SOLUBLE-FORMAZAN ASSAY FOR HIV-1 CYTOPATHIC EFFECTS - APPLICATION TO HIGH-FLUX SCREENING OF SYNTHETIC AND NATURAL-PRODUCTS FOR AIDS-ANTIVIRAL ACTIVITY
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DOI：
10.1093/jnci/81.8.577
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发表时间：
1989-04-19
影响因子：
10.3
通讯作者：
BOYD, MR
中科院分区：
文献类型：
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作者：
WEISLOW, OS;KISER, R;BOYD, MR
我们建立了一种有效的、最安全的微量培养方法，用于快速、方便地测定人类免疫缺陷病毒（HIV-1）对人类淋巴母细胞或其他合适的宿主细胞的体外细胞病变效应。本试验方法适用于评价药物对无细胞HIV-2或与HIV-1慢性感染的H9细胞共培养直接在培养的宿主细胞中诱导的体外感染的影响。该检测使用新开发的四唑试剂，该试剂通过活细胞代谢还原，产生可通过常规比色技术测量的可溶性有色甲瓒产物。这种简单的微量测定最大限度地减少了其他测定方法通常需要的平板操作次数，加上计算机化的数据收集和分析，有助于大规模筛选潜在抗病毒活性的药物。为了支持和加强新的抗HIV-1药物的发现，美国国家癌症研究所正在为全球研究人员提供机会，以提交新的候选药物，用于使用这种方法进行抗HIV-1筛选。
We have developed an effective and optimally safe microculture method for rapid and convenient assay of the in vitro cytopathic effects of human immunodeficiency virus (HIV-1) on human lymphoblastoid or other suitable host cells. The assay procedure is applicable to the evaluation of drug effects on in vitro infections induced directly in cultured host cells by cell-free HIV-2 or by coculture with H9 cells chronically infected with HIV-1. The assay uses a newly developed tetrazolium reagent that is metabolically reduced by viable cells to yield a soluble, colored formazan product measurable by conventional colorimetric techniques. This simple microassay minimizes the number of plate manipulations typically required with other assay methods and, coupled with computerized data collection and analysis, facilitates large-scale screening of agents for potential antiviral activity. To support and enhance the discovery of new anti-HIV-1 agents, the National Cancer Institute is offering investigators worldwide the opportunity to submit new candidate agents for anti-HIV-1 screening with this method.