Long-read genome assemblies for the study of chromosome expansion: Drosophila kikkawai , Drosophila takahashii , Drosophila bipectinata , and Drosophila ananassae.
Long-read genome assemblies for the study of chromosome expansion: Drosophila kikkawai , Drosophila takahashii , Drosophila bipectinata , and Drosophila ananassae.
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用于染色体扩展研究的长读基因组组装：Drosophila kikkawai、Drosophila takahashii、Drosophila bipectinata 和 Drosophila ananassae。
DOI：
10.1101/2023.05.22.541758
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Ellison,ChristopherE
中科院分区：
文献类型：
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作者：
Leung,Wilson;Torosin,Nicole;Cao,Weihuan;Reed,LauraK;Arrigo,Cindy;Elgin,SarahCR;Ellison,ChristopherE
流式细胞术对果蝇物种间基因组大小的估计显示出 3 倍的差异，范围从果蝇 Mercatorum 的约 127 Mb 到果蝇 Cyrtoloma 的约 400 Mb。然而，Muller F 元件（与果蝇第四条染色体直系同源）的组装部分在大小上显示出近 14 倍的变化，范围从 ∼1.3 Mb 到 > 18 Mb。在这里，我们展示了 4 个果蝇物种的染色体水平长读基因组组装，其扩展的 F 元件大小范围为 2.3 至 20.5 Mb。每个 Muller 元件在每个组件中都作为单个支架存在。这些组装将使人们对染色体尺寸扩张的进化原因和后果有新的认识。
Flow cytometry estimates of genome sizes among species ofDrosophilashow a 3-fold variation, ranging from ∼127 Mb inDrosophila mercatorumto ∼400 Mb inDrosophila cyrtoloma. However, the assembled portion of the Muller F element (orthologous to the fourth chromosome inDrosophila melanogaster) shows a nearly 14-fold variation in size, ranging from ∼1.3 Mb to >18 Mb. Here, we present chromosome-level long-read genome assemblies for 4Drosophilaspecies with expanded F elements ranging in size from 2.3 to 20.5 Mb. Each Muller element is present as a single scaffold in each assembly. These assemblies will enable new insights into the evolutionary causes and consequences of chromosome size expansion.