[3H]quinuclidinyl benzilate binding to the human lung muscarinic receptor.
[3H]quinuclidinyl benzilate binding to the human lung muscarinic receptor.
复制标题
[3H]苯苯甲酸奎宁环酯与人肺毒蕈碱受体结合。
DOI：
10.1016/0006-2952(88)90193-1
复制
发表时间：
1988
影响因子：
5.8
通讯作者：
Casale,TB
中科院分区：
文献类型：
--
作者：
Joad,JP;Casale,TB
材料与方法：牛血清白蛋白（BSAt）、东莨菪碱、阿托品、氧tremorine、methachhne chloride、carbachol、组胺二磷酸和D、l -异丙肾上腺素购自Sigma， St. Louis， MO；蔗糖和氯化镁购自Fisher Scientific Co， Fair Lawn， NJ；Tris是从印第安纳州印第安纳波利斯的勃林格曼海姆生物化学公司购买的；Dulbecco培养基购自宾夕法尼亚州丹佛市Hazieton；苯磺酸喹喹啉基（ONB）是罗氏公司（Hoffmann-La Roche）的产品。新泽西州,新泽西;mand ' [3H] QNB （39.4 Ci/mmol）购自马萨诸塞州波士顿的New England Nuclear公司。
Materials and methodsReagents. Bovine serum albumin (BSAt), scopolamine, atropine, oxotremorine, methachohne chloride, carbachol, histamine diphosphate and D, L-isoproterenol were purchased from Sigma, St. Louis, MO; sucrose and magnesium chloride were purchased from the Fisher Scientific Co., Fair Lawn, NJ; Tris was purchased from Boehringer Mannheim Biochemicals, Indianapolis, IN; Dulbecco’s medium was purchased from Hazieton, Denver, PA; quinuclidinyl benzilate (ONB) was a eift from Hoffmann-La Roche. Nutley, NJ; mand’[3H] QNB (39.4 Ci/mmol) was purchased from New England Nuclear, Boston, MA.