Small single-copy region of plastid DNA in the non-photosynthetic angiosperm Epifagus virginiana contains only two genes. Differences among dicots, monocots and bryophytes in gene organization at a non-bioenergetic locus.

Small single-copy region of plastid DNA in the non-photosynthetic angiosperm Epifagus virginiana contains only two genes. Differences among dicots, monocots and bryophytes in gene organization at a non-bioenergetic locus.
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非光合作用被子植物 Epifagus virginiana 质体 DNA 的小单拷贝区域仅包含两个基因。

DOI:
10.1016/0022-2836(92)90718-y
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发表时间:
1992
影响因子:
5.6
通讯作者:
Palmer,JD
Palmer,JD
中科院分区:
生物学2区
文献类型:
--
作者:
Wolfe,KH;Morden,CW;Palmer,JD

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我们已经确定了一个7 kb(1 kb = 103个碱基对)区域的核苷酸序列,该区域包括非光合作用的寄生开花植物Epifagus virginiana质体基因组的整个小单拷贝区域(SSC)。SSC(4.8 kb)是相当小的光合植物,由于所有的光合作用,叶绿体呼吸和核糖体蛋白基因的完全删除。只剩下两个基因:1738个密码子的蛋白质基因,其产物不太可能参与生物能量过程和亮氨酸tRNA基因(trnLUAG)。这两个基因跨越反向重复序列和SSC之间的连接,结果是重复序列的末端20个碱基对在两个方向上转录,并作为tRNA基因的3 ′端和f1738的内部片段发挥作用。我们发现烟草质体DNA中与Epifagus orf 1738同源的区域含有一个1901个密码子的开放阅读框(ORF),而不是原来报道的1244、273和228个密码子的三个ORF。然而,我们证实,branditeMarchantia的等效区域包含两个基因(1068和464密码子),分别对应于双子叶植物蛋白的N和C-末端部分。与此相反,水稻质体DNA含有一个严重截短的假基因在这个位点。
We have determined the nucleotide sequence of a 7 kb (1 kb = 103base-pairs) region that includes the entire small single-copy region (SSC) of the plastid genome ofEpifagus virginiana, a non-photosynthetic, parasitic flowering plant. The SSC (4.8 kb) is considerably smaller than those of photosynthetic plants due to the complete deletion of all photosynthetic, chlororespiratory and ribosomal protein genes. This leaves only two genes: a protein gene of 1738 codons whose product is unlikely to be involved in bioenergetic processes and a leucine tRNA gene (trnLUAG). Both genes span junctions between the inverted repeat and the SSC, with the consequence that the terminal 20 base-pairs of the repeat is transcribed in both directions and functions both as the 3′ end of the tRNA gene and as an internal segment oforf1738. We find that the region of tobacco plastid DNA homologous toEpifagus orf1738contains a single open reading frame (ORF) of 1901 codons rather than the three ORFs of 1244, 273 and 228 codons originally reported. However, we confirm that the equivalent region of the bryophyteMarchantiacontains two genes (1068 and 464 codons) corresponding to the N and C-terminal portions of the dicot protein. In contrast, rice plastid DNA contains a severely truncated pseudogene at this locus.
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