GDEE ANTAGONISM OF IONTOPHORETIC AMINO-ACID EXCITATIONS IN INTACT HIPPOCAMPUS AND IN HIPPOCAMPAL SLICE PREPARATION
GDEE ANTAGONISM OF IONTOPHORETIC AMINO-ACID EXCITATIONS IN INTACT HIPPOCAMPUS AND IN HIPPOCAMPAL SLICE PREPARATION
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DOI：
10.1016/0006-8993(76)90594-1
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发表时间：
1976-01-01
期刊：
影响因子：
2.9
通讯作者：
LYNCH, GS
中科院分区：
文献类型：
--
作者：
SPENCER, HJ;GRIBKOFF, VK;LYNCH, GS
谷氨酸二乙酯（GDEE）可逆地拮抗兴奋谷氨酸和天冬氨酸产生的，但不是那些产生的乙酰胆碱时，应用离子电渗给大鼠CA 1海马神经元在戊蒽（甲氧基荧烷）麻醉大鼠和CA 1神经元在体外切片制备。GDEE似乎没有区分谷氨酸和天冬氨酸产生的兴奋，似乎是一个更有效的拮抗剂比以前报道的。CA 1细胞对乙酰胆碱非常敏感; 5-50 nA足以产生明显的氨基酸样兴奋，这与乙酰胆碱的pH无关。完整动物和体外切片制备物对所用物质的反应性质几乎相同。体外技术的描述见附录。
Glutamic acid diethylester (GDEE) reversibly antagonized excitations produced by glutamate and aspartate but not those produced by acetylcholine when applied iontophoretically to rat CA1 hippocampal neurons in penthrane (methoxyfluorane) anesthetized rats and to CA1 neurons in in vitro slice preparations. GDEE did not appear to differentiate between the excitations produced by glutamate and aspartate and appeared to be a more potent antagonist than has previously been reported. CA1 cells were remarkably sensitive to acetylcholine; 5-50 nA being sufficient to produce marked amino acid-like excitations, which were unrelated to the pH of the acetylcholine. The nature of the responses to applied substances was virtually identical between the intact animal and the in vitro slice preparation. A description of the in vitro technique is given in an appendix.