Arabinogalactan isolated from cowshed dust extract protects mice from allergic airway inflammation and sensitization
Arabinogalactan isolated from cowshed dust extract protects mice from allergic airway inflammation and sensitization
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DOI：
10.1016/j.jaci.2010.05.011
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发表时间：
2010-09-01
影响因子：
14.2
通讯作者：
Bufe, Albrecht
中科院分区：
文献类型：
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作者：
Peters, Marcus;Kauth, Marion;Bufe, Albrecht
背景：牛舍尘提取物（CDE）是免疫调节物质的来源。我们以前已经表明，这种物质保护实验过敏性疾病的小鼠模型asthma.Objective：本研究的目的是确定免疫调节分子的提取物中的粉尘过敏保护的农业环境。方法：采用层析和特异性试剂沉淀法从CDE和植物中分离多糖。然后通过核磁共振光谱对多糖进行表征。随后，过敏保护潜力的分离多糖进行了测试，在小鼠模型asthma.Results：作者表明，植物阿拉伯半乳聚糖中含有高浓度的CDE。这种阿拉伯半乳聚糖的来源是饲料，特别是被称为草地看麦娘（Alopecurus pratensis）的常见草物种。用草阿拉伯半乳聚糖处理小鼠树突状细胞导致自分泌IL-10产生。有趣的是，这些树突状细胞不能诱导过敏性免疫反应。此外，在过敏性哮喘的小鼠模型中，鼻内施用草阿拉伯半乳聚糖保护小鼠免于发生特应性致敏、过敏性气道炎症和气道高反应性。这种过敏保护作用是针对草阿拉伯半乳聚糖的，因为来自阿拉伯树胶和落叶松的阿拉伯半乳聚糖的对照实验显示，这些分子不显示过敏保护特性。这可能是因为结构的差异，因为我们能够通过核磁共振光谱表明，虽然他们主要是由阿拉伯糖和半乳糖，分子的结构不同Conclusions：作者的结论是，草阿拉伯半乳聚糖是重要的免疫调节物质，有助于保护过敏性气道炎症，气道高反应性，特应性致敏小鼠哮喘模型。（J Allergy Clin Immunol 2010;126：648-56.）
Background: Extract from cowshed dust (CDE) is a source of immunomodulating substances. We have previously shown that such substances protect from experimental allergic disorders in a mouse model of asthma.Objective: The objective of this study was to identify immunomodulatory molecules in extracts of dust from an allergy protective farming environment. Methods: Polysaccharides were isolated from CDE and plants by chromatography and precipitation with specific reagents. Polysaccharides were then characterized by nuclear magnetic resonance spectroscopy. Subsequently, the allergy-protective potential of isolated polysaccharides was tested in a mouse model of asthma.Results: The authors demonstrate that plant arabinogalactans are contained in CDE in high concentrations. The source of this arabinogalactan is fodder, in particular a prevalent grass species known as Alopecurus pratensis. Treatment of murine dendritic cells with grass arabinogalactan resulted in autocrine IL-10 production. Interestingly, these dendritic cells were not able to induce an allergic immune response. Furthermore, intranasal application of grass arabinogalactan protected mice from developing atopic sensitization, allergic airway inflammation and airway hyperreactivity in a mouse model of allergic asthma. This allergy-protective effect is specific for grass arabinogalactan because control experiments with arabinogalactan from gum arabic and larch revealed that these molecules do not show allergy-protective properties. This is likely because of structural differences because we were able to show by nuclear magnetic resonance spectroscopy that although they are predominantly composed of arabinose and galactose, the molecules differ in structure.Conclusions: The authors conclude that grass arabinogalactans are important immunomodulatory substances that contribute to the protection from allergic airway inflammation, airway hyperresponsiveness, and atopic sensitization in a mouse model of asthma. (J Allergy Clin Immunol 2010;126:648-56.)