Innovative method for the enrichment of high-polarity bioactive molecules present at low concentrations in complex matrices.
Innovative method for the enrichment of high-polarity bioactive molecules present at low concentrations in complex matrices.
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DOI：
10.1002/jssc.201601193
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发表时间：
2017
影响因子：
3.1
通讯作者：
Qing-shan Liu;Jie He;Wen-Bin Zhou;Yu-Long Gu;Huoqiang Huang;Keqin Li;X. Yin
中科院分区：
文献类型：
--
作者：
Qing-shan Liu;Jie He;Wen-Bin Zhou;Yu-Long Gu;Huoqiang Huang;Keqin Li;X. Yin
人参皂苷Rg 1是一种有价值的生物活性分子，但其高极性和复杂混合物中的低浓度使得将人参皂苷Rg 1与具有相似结构的其他皂苷分离成为一个挑战，导致提取效率低。具有高选择性和高吸附性的Rg 1分子印迹聚合物的成功开发为活性化合物的富集提供了一种改进的方法。本工作采用沉淀聚合法和表面印迹聚合法制备了分子印迹聚合物。吸附能力的比较表明，更高的吸附的表面分子印迹聚合物制备的表面印迹聚合物，46.80 mg/g，相比，观察到的27.74 mg/g的分子印迹聚合物制备的沉淀聚合。因此，为了提高对强极性Rg 1的吸附能力，表面印迹聚合是制备Rg 1分子印迹聚合物的一种上级技术。将所制备的表面分子印迹聚合物作为固相萃取柱，对人参茶和人参总提取物中人参皂苷Rg 1及其类似物进行了定向富集。与C18固相萃取柱相比，表面分子印迹聚合物柱表现出更高的富集效率和更好的选择性。总的来说，开发了一种新的创新方法来有效富集复杂基质中低浓度存在的高极性生物活性分子。
Ginsenoside Rg1 is a valuable bioactive molecule but its high polarity and low concentration in complex mixtures makes it a challenge to separate Ginsenoside Rg1 from other saponins with similar structures, resulting in low extraction efficiency. The successful development of effective Rg1 molecularly imprinted polymers that exhibit high selectivity and adsorption may offer an improved method for the enrichment of active compounds. In this work, molecularly imprinted polymers were prepared with two different methods, precipitation polymerization or surface imprinted polymerization. Comparison of the adsorption abilities showed higher adsorption of the surface molecularly imprinted polymers prepared by surface imprinted polymerization, 46.80 mg/g, compared to the 27.74 mg/g observed for the molecularly imprinted polymers prepared by precipitation polymerization. Therefore, for higher adsorption of the highly polar Rg1, surface imprinted polymerization is a superior technique to make Rg1 molecularly imprinted polymers. The prepared surface molecularly imprinted polymers were tested as a solid-phase extraction column to directionally enrich Rg1 and its analogues from ginseng tea and total ginseng extracts. The column with surface molecularly imprinted polymers showed higher enrichment efficiency and better selectivity than a C18 solid-phase extraction column. Overall, a new, innovative method was developed to efficiently enrich high-polarity bioactive molecules present at low concentrations in complex matrices.