Root diffusion barrier control by a vasculature-derived peptide binding to the SGN3 receptor
Root diffusion barrier control by a vasculature-derived peptide binding to the SGN3 receptor
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DOI：
10.1126/science.aaj1562
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发表时间：
2017-01-20
期刊：
影响因子：
56.9
通讯作者：
Geldner, Niko
中科院分区：
文献类型：
--
作者：
Doblas, Veronica G.;Smakowska-Luzan, Elwira;Geldner, Niko
根内皮层通过形成称为凯氏带的环状浸渍形成其细胞外扩散屏障。负责其建立的因子是SCHENGEN 3/GASSHO 1（SGN 3/GSO 1）受体样激酶。其功能的丧失导致不连续的凯氏带。SGN 3还介导其他凯氏带突变体的内胚层过度木质化。然而，没有配体，SGN 3功能仍然难以捉摸。在这里，我们报告，申根2（sgn 2）是有缺陷的酶硫酸化肽配体。在此观察的基础上，我们确定了两个中柱表达的肽（卡斯帕里安带完整性因子，CIF 1/2），补充sgn 2在纳摩尔浓度和诱导卡斯帕里安带错误定位以及过度lignation-all依赖于SGN 3。与重组SGN 3直接结合的肽鉴定这些肽为SGN 3配体。我们推测CIF 1/2-SGN 3是屏障监视系统的一部分，其进化是为了保证超细胞凯氏带网络的有效密封。
The root endodermis forms its extracellular diffusion barrier by developing ringlike impregnations called Casparian strips. A factor responsible for their establishment is the SCHENGEN3/GASSHO1 (SGN3/GSO1) receptor-like kinase. Its loss of function causes discontinuous Casparian strips. SGN3 also mediates endodermal overlignification of other Casparian strip mutants. Yet, without ligand, SGN3 function remained elusive. Here we report that schengen2 (sgn2) is defective in an enzyme sulfating peptide ligands. On the basis of this observation, we identified two stele-expressed peptides (CASPARIAN STRIP INTEGRITY FACTORS, CIF1/2) that complement sgn2 at nanomolar concentrations and induce Casparian strip mislocalization as well as overlignification-all of which depend on SGN3. Direct peptide binding to recombinant SGN3 identifies these peptides as SGN3 ligands. We speculate that CIF1/2-SGN3 is part of a barrier surveillance system, evolved to guarantee effective sealing of the supracellular Casparian strip network.