Bridging molecules are secreted from the skeletal muscle and potentially regulate muscle differentiation
Bridging molecules are secreted from the skeletal muscle and potentially regulate muscle differentiation
复制标题
DOI：
10.1016/j.bbrc.2019.11.010
复制
发表时间：
2020-01-29
影响因子：
3.1
通讯作者：
Sato, Ryuichiro
中科院分区：
文献类型：
--
作者：
Chikazawa, Miho;Shimizu, Makoto;Sato, Ryuichiro
肌肉生肌是肌肉发育和恢复的重要步骤。在肌肉融合过程中，多种分子被认为是正常肌管形成所必需的。乳脂肪球-EGF 因子 8 (MFG-E8) 和 Gas6 是磷脂酰丝氨酸识别桥接分子，主要由免疫细胞分泌。在这项研究中，我们证实这些分子是由 C2C12 细胞表达和分泌的。小鼠肌肉和卫星细胞也表达这些分子。 MFG-E8 在未分化和分化的 C2C12 细胞中高度表达和分泌。我们观察到，与未分化细胞相比，MFG-E8 和 Gas6 与分化的 C2C12 细胞表面的结合更多。此外，重组 MFG-E8 的处理上调了 C2C12 细胞生肌过程中生肌基因的表达并抑制了细胞凋亡。在本文中，我们讨论了骨骼肌中表达和分泌的新型功能分子的存在。这项研究的结果表明桥接分子是肌生成或其他肌肉反应的决定因素之一。 (C) 2019 年由爱思唯尔公司出版
Muscle myogenesis is an essential step for muscle development and recovery. During muscle fusion, multiple molecules are thought to be necessary for the formation of normal myotubes. Milk fat globule-EGF factor 8 (MFG-E8) and Gas6 are phosphatidylserine-recognizing bridging molecules that are secreted mainly from immune cells. In this study, we confirmed that these molecules are expressed and secreted from C2C12 cells. Mouse muscle and satellite cells also expressed these molecules. MFG-E8 was highly expressed and secreted in both undifferentiated and differentiated C2C12 cells. We observed that MFG-E8 and Gas6 were bound to the surface of differentiated C2C12 cells more compared with undifferentiated cells.Additionally, the treatment of recombinant MFG-E8 upregulated expression of myogenic genes and suppressed apoptosis during myogenesis in C2C12 cells. In this paper, we discuss the presence of novel functional molecules expressed and secreted in the skeletal muscle. The results of this study suggest that bridging molecules are one of the determinants of myogenesis or other muscle responses. (C) 2019 Published by Elsevier Inc.