Short report: Genetic heterogeneity of Japanese encephalitis virus assessed via analysis of the full-length genome sequence of a Korean isolate
Short report: Genetic heterogeneity of Japanese encephalitis virus assessed via analysis of the full-length genome sequence of a Korean isolate
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DOI：
10.4269/ajtmh.2001.65.388
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发表时间：
2001-10-01
影响因子：
3.3
通讯作者：
Cho, HW
中科院分区：
文献类型：
--
作者：
Nam, JH;Chae, SL;Cho, HW
我们测定了日本脑炎病毒(JEV)韩国分离株K94P05的全基因组序列。序列分析表明，由于K94P05的3‘非编码区缺失，K94P05株10,963个核苷酸的RNA基因组比其他乙脑病毒分离株的基因组短13或14个核苷酸。与其他乙型脑炎病毒分离株的核苷酸序列比较，同源性为89.0-89.6%，推导的氨基酸序列同源性在96.4-97.3%之间。与其他乙型脑炎病毒分离株相比，K94P05开放阅读框终止密码子下游约60个核苷酸的区域显示出较高的序列变异性。K94P05在用全长基因组序列建立的系统发育树中形成了一个独特的类群。交叉中和研究表明，针对韩国分离株的多克隆抗体对韩国分离株的中和效果是针对Nakayama-NIH的抗体的3倍。这些发现表明，韩国乙脑病毒K94P05在基因和抗原性上与其他亚洲乙脑病毒分离株不同。
We determined the full-length genome sequence of Japanese encephalitis virus (JEV) K94P05 isolated in Korea. Sequence analysis showed that the 10,963-nucleotide-long RNA genome of K94P05 was 13 or 14 nucleotides shorter than the genome of other JEV isolates because of a deletion in the 3 ' noncoding region of K94P05. Compared with sequences of other JEV isolates, the full-length nucleotide sequence showed 89.0-89.6% homology, and the deduced amino acid sequence showed between 96.4-97.3% homology. A region of similar to 60 nucleotidcs immediately downstream of the open reading frame stop codon of K94P05 showed high sequence variability as compared with other JEV isolates. K94P05 formed a distinct group within a phylogenetic tree established with the full-length genome sequences. Cross- neutralization studies showed that polyclonal antibodies to Korean isolates were 3 times better at neutralizing the Korean isolates than antibodies to Nakayama-NIH. These findings suggest that Korean JEV K94P05 is genetically and antigenically distinct from other Asian JEV isolates.