Flavobacterium ammonificans sp. nov. and Flavobacterium ammoniigenes sp. nov., ammonifying bacteria isolated from surface river water
Flavobacterium ammonificans sp. nov. and Flavobacterium ammoniigenes sp. nov., ammonifying bacteria isolated from surface river water
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DOI：
10.1099/ijsem.0.005307
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发表时间：
2022-01-01
影响因子：
2.8
通讯作者：
Suda, Wataru
中科院分区：
文献类型：
--
作者：
Watanabe, Keiji;Kitamura, Tatsumi;Suda, Wataru
三个有氧运动。从地表河水（日本琦玉县）中分离出革兰氏染色阴性、非运动、杆状细菌，命名为菌株SHINM 13（T）、GENT 5（T）和GENT 11。SHINM 13（T）和GENT 11为过氧化氢酶阳性。而GENT 5（T）为阴性。基于16 S rRNA基因（1341 bp）或40个标记基因（34，513 bp）序列的系统发育分析显示，这些菌株在黄杆菌属内形成了不同的系统发育谱系。3个菌株165 rRNA基因序列的相似性为99.3-99.6%。SHINM 13（T）与GENT 11的同源性（OrthoANI）和dDDH值分别为96.56%和82.1%，与GENT 5（T）的同源性分别为83.46%和52.9%。主要的细胞脂肪酸是C-15：1 omega 6c、iso-C-15：0、iso-C（15：1）G、anteiso-C-15：0和iso-C-15：0 3-OH。主要极性脂质为磷脂酰乙醇胺。SHINM 13（T）和GENT 5（T）的呼吸醌主要为甲基萘醌-6（MK-6），其DNA G+C含量分别为34.4和35.1mol%。三个分离株的基因组测序显示基因组大小为2.26-2.40 Mbp。此外，所有三个菌株在细胞生长过程中将溶解的有机氮转化为铵。基于表型和系统发育分析的结果，菌株SHINM 13（T）和GENT 11和GENT 5（T）代表黄杆菌属中的两个不同的新物种，为此命名为黄杆菌氨化亚种（Flavobacterium ammonificans sp. nov.）（模式菌株SHINM 13（T）=JCM 34684（T）=NCIMB 15379（T））和产氨黄杆菌新种（模式菌株GENT 5（T）=JCM 32249（T）=NCIMB 15380（T））。
Three aerobic. Gram-stain-negative, non-motile, rod-shaped bacteria, designated as strains SHINM13(T), GENT5(T) and GENT11 were isolated from surface river water (Saitama Prefecture, Japan). SHINM13(T) and GENT11 were positive for catalase. whereas GENT5(T) was negative. Phylogenetic analyses based on the 16S rRNA gene (1341 bp) or 40 marker gene (34,513 bp) sequences revealed that the strains formed distinct phylogenetic lineages within the genus Flavobacterium. The three strains shared 99.3-99.6% 165 rRNA gene sequence similarity among each other. The average nucleotide identity by orthology (OrthoANI) and digital DNA-DNA hybridization (dDDH) values between strains SHINM13(T) and GENT11 were 96.56 and 82.1%, respectively, and those between SHINM13(T) and GENT5(T) were 83.46% and 52.9 %, respectively. The major cellular fatty acids were C-15:1 omega 6c, iso-C-15:0, iso-C(15:1)G, anteiso-C-15:0 and iso-C-15:0 3-OH. The major polar lipid was phosphatidylethanolamine. SHINM13(T) and GENT5(T) contained menaquinone-6 (MK-6) as the predominant respiratory quinone, and their DNA G+C contents were 34.4 and 35.1 mol%, respectively. Genome sequencing of the three isolates revealed a genome size of 2.26-2.40 Mbp. Furthermore, all three isolates converted dissolved organic nitrogen to ammonium during cell growth. On the basis of the results of phenotypic and phylogenetic analyses, strains SHINM13(T) and GENT11 and GENT5(T) represent two distinct novel species in the genus Flavobacterium, for which the names Flavobacterium ammonificans sp. nov. (type strain SHINM13(T) =JCM 34684(T) =NCIMB 15379(T)) and Flavobacterium ammoniigenes sp. nov. (type strain GENT5(T) =JCM 32249(T)=NCIMB 15380(T)) are proposed.