INTERACTIONS OF MONO-VALENT CATIONS WITH PHOSPHATIDYLSERINE BILAYER-MEMBRANES
INTERACTIONS OF MONO-VALENT CATIONS WITH PHOSPHATIDYLSERINE BILAYER-MEMBRANES
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DOI：
10.1021/bi00278a018
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发表时间：
1983-01-01
期刊：
影响因子：
2.9
通讯作者：
SHIPLEY, GG
中科院分区：
文献类型：
--
作者：
HAUSER, H;SHIPLEY, GG
在对水合二酰基磷脂酰丝氨酸的结构和向热性质的描述之后（豪瑟，H.，Paltauf，F. Shipley，G. G.，1982），研究了单价阳离子对磷脂酰丝氨酸（PS）的相同同源系列的行为的影响。利用差示扫描量热法和X-射线衍射，二酰基磷脂酰丝氨酸的铵盐[二-C12（DLPS [二月桂酰磷脂酰丝氨酸]）、二-C14（DMPS [二肉豆蔻酰磷脂酰丝氨酸]）、二-C16（DPPS [二棕榈酰磷脂酰丝氨酸]）和二-C18（DSPS [二硬脂酰磷脂酰丝氨酸]）]显示出形成显示出链长依赖性凝胶的水合双层膜结构。液晶转变此外，高达1 M浓度的NaCl，KCl，RbCl和CsCl产生相对较小的变化，DMPS双层结构的稳定性。例如，分散在摩尔浓度增加的NaCl或KCl（0- 1.5M）中的NH 4 +-二肉豆蔻酰-PS（NH 4 +-DMPS）显示转变温度从39 ℃仅小的S形增加。到42度。或43 °。C时，转变焓（Δ H约）变化很小或没有变化。7.4 kcal/mol）。50wt% NH 4 +-DMPS水分散体的X射线衍射显示层状双层重复距离d = 107。以及在1/4.2（埃- 1)六方凝胶烃链堆积的特征。在增加的NaCl摩尔浓度的存在下，双层周期性逐渐减少，因为Na+屏蔽带负电荷的双层表面。62埃的极限值。到达. apprx。0.5 M NaCl。1/4.2（Δ G-Δ G）没有变化。1)反射，表明烃链堆积没有发生显著变化。对于DLPS、DPPS和DSPS证明了类似的行为。添加氯化锂产生渐进结晶的DLPS，DMPS，和DPPS双层所揭示的存在下，在广角区域中的一些X-射线衍射线，和双层周期性降低。在1 M LiCl时，NH 4 +-DMPS的双层周期性降低到51埃，和Li+-PS络合物都在高得多的温度（Δ T = 40 - 45 ℃）下表现出从结晶双层形式到液晶相的转变。50.degree. C），并且与不含Li+的PS相比，与转变相关的焓变大约加倍。与其它一价阳离子相比，无Li+的PS.与其他一价阳离子相比，Li+诱导PS双层等温结晶，Li+诱导PS双层等温结晶，烃链采用比通常水合凝胶状态的六方排列更有序的堆积模式。L+在PS双层中产生的结构变化与Mg 2+和Ca 2+产生的结构变化相似，这一观察结果可能与Li+在治疗[人类]躁狂抑郁症中的药理学用途有关。
Following a description of the structure and thermotropic properties of hydrated diacylphosphatidylserines (Hauser, H., Paltauf, F. and Shipley, G. G., 1982), the effect of monovalent cations on the behavior of the same homologous series of phosphatidylserine (PS) was examined. With the utilization of differential scanning calorimetry and X-ray diffraction, the ammonium salts of diacylphosphatidylserines [di-C12 (DLPS [dilauroylphosphatidylserine]), di-C14 (DMPS [dimyristolphosphatidylserine]), di-C16 (DPPS [dipalmitoylphosphatidylserine]) and di-C18 (DSPS [distearoylphosphatidylserine])] were shown to form hydrated bilayer membrane structures exhibiting chain length dependent gel .fwdarw. liquid-crystal transitions. Addition of up to 1 M concentrations of NaCl, KCl, RbCl and CsCl produced relatively minor changes in DMPS bilayer structure of stability. For example, NH4+-dimyristoyl-PS (NH4+-DMPS) dispersed in NaCl or KCl of increasing molarity, 0-1.5 M, shows only a small, sigmoidal increase in transition temperature from 39.degree. to 42.degree. or 43.degree. C, with little or no change in transition enthalpy (.DELTA.H .simeq. 7.4 kcal/mol). X-ray diffraction of 50 wt% aqueous dispersions of NH4+-DMPS shows a lamellar bilayer repeat distance d = 107 .ANG. and a sharp reflection at 1/4.2 (.ANG.-1) characteristic of the hexagonal gel hydrocarbon chain packing. In the presence of increasing NaCl molarity, the bilayer periodicity is progressively reduced as Na+ shields the negatively charged bilayer surface. A limiting value of 62 .ANG. is reached at .apprx. 0.5 M NaCl. No changes occur to the 1/4.2 (.ANG.-1) reflection, showing that no significant change occurs in the hydrocarbon chain packing. Similar behavior is demonstrated for DLPS, DPPS and DSPS. Addition of LiCl produces progressive crystallization of DLPS, DMPS, and DPPS bilayers as revealed by the presence of a number of X-ray diffraction lines in the wide-angle region, and the bilayer periodicity is reduced. At 1 M LiCl, the bilayer periodicity of NH4+-DMPS is reduced to 51 .ANG., and the Li+-PS complexes all exhibit transitions from a crystalline bilayer form to liquid-crystal phase at much higher temperatures (.DELTA.T = 40.degree.-50.degree. C), and the enthalpy change associated with the transition approximately doubles compared to Li+-free PS. In contrast to the other monovalent cations, Li+-free PS. In contrast to the other monovalent cations, Li+ induces an isothermal crystallization of PS bilayers, Li+ induces an isothermal crystallization of PS bilayers, the hydrocarbon chains adopting a more ordered packing mode than the hexagonal arrangement of the usual hydrated gel state. The structural changes produced in PS bilayers by L+ are similar to those produced by Mg2+ and Ca2+, an observation perhaps pertinent to the pharmacological use of Li+ in the treatment of [human] manic-depressive illness.