Development of Cu nanoflowers modified the flexible needle-type microelectrode and its application in continuous monitoring glucose in vivo
Development of Cu nanoflowers modified the flexible needle-type microelectrode and its application in continuous monitoring glucose in vivo
复制标题
铜纳米花修饰柔性针型微电极的研制及其在体内血糖连续监测中的应用
DOI：
10.1016/j.bios.2018.03.024
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发表时间：
2018-07-01
影响因子：
12.6
通讯作者：
Guo, Yi
中科院分区：
文献类型：
--
作者：
Fang, Yuxin;Wang, Shenjun;Guo, Yi
本研究开发了一种基于柔性集成电极的微创葡萄糖微生物传感器，用于连续监测体内葡萄糖。这是通过涂覆针型微电极与铜纳米花，全氟磺酸，葡萄糖氧化酶（GOD）和聚氨酯（PU）膜，成功地制备与层层沉积。铜纳米材料具有较大的比表面积和电催化活性，可用于葡萄糖的检测。聚氨酯层作为质量传输限制膜显着提高了传感器的线性和稳定性。该传感器的线性范围为0.20 mM，灵敏度为42.38 nA mM（-1）（相关系数r（2）为0.99），响应时间小于15 s。使用麻醉大鼠的体内植入实验显示出对血糖浓度变化的良好实时响应。传感器输出的变化趋势与血糖仪的变化趋势一致。总体而言，结果支持该微传感器用于测量体内葡萄糖的快速变化的适用性。这项工作为与糖尿病管理以及其他医疗诊断相关的植入式设备应用提供了一种有前途的方法。
A minimally invasive glucose microbiosensor based the flexibly integrated electrode for continuous monitoring glucose in vivo has been developed in this study. This was achieved by coating needle-type microelectrode with Cu nanoflowers, nafion, glucose oxidase (GOD) and polyurethane (PU) membranes, successfully prepared with layer-by-layer deposition. The Cu nanomaterials provided a large specific surface area and electrocatalytic activity for glucose detection. The PU layers as mass-transport limiting membranes significantly enhanced the linearity and stability of sensors. The resulting biosensor exhibited a wide linear range of 020 mM, with a good sensitivity of 42.38 nA mM(-1) (correlation coefficient r(2) was 0.99) and a fast response time of less than 15 s. In vivo implantable experiments using anesthetized rats showed excellent real-time response to the variation of blood glucose concentration. And the variation tendency of sensor output was consistent with that using the glucose meter. Overall, the results supported the suitability of this microsensor for measuring rapid changes of glucose in vivo. This work offers a promising approach in implantable device applications related to diabetes management as well as other medical diagnosis.