Quercetin improve ischemia/reperfusion-induced cardiomyocyte apoptosis in vitro and in vivo study via SIRT1/PGC-1 signaling
Quercetin improve ischemia/reperfusion-induced cardiomyocyte apoptosis in vitro and in vivo study via SIRT1/PGC-1 signaling
复制标题
槲皮素通过 SIRT1/PGC-1α 信号传导改善缺血/再灌注诱导的心肌细胞凋亡的体外和体内研究
DOI：
10.1002/jcb.28255
复制
发表时间：
2019-06-01
影响因子：
4
通讯作者：
Yang, Jian
中科院分区：
文献类型：
--
作者：
Tang, Jiayou;Lu, Linhe;Yang, Jian
目的探讨槲皮素在体外和体内对缺血再灌注诱导心肌细胞凋亡的影响。方法将小鼠细胞分为5组：模型对照组（MC）为缺血再灌注（I/R）模型组；DL组在MC组基础上加用25mL/L槲皮素；DM组在MC组基础上加用50ml/L槲皮素；DH组在MC组基础上加用100mL/L槲皮素；Meto组在MC组基础上加用美托洛尔治疗。在体实验中，将大鼠分为5组：MC组为I/R模型组；DL组给予槲皮素25mg/kg；DM组给予50mg/kg槲皮素；DM组给予槲皮素100mg/kg；Meto组以Meto为阳性药物治疗。结果槲皮素处理组（DL组、DM组、DH组）细胞凋亡率较MC组明显降低。槲皮素处理组的沉默信息调节因子1 （SIRT1）、过氧化物酶体增殖物激活受体-辅助激活因子1 （PGC-1）和Bcl-2蛋白表达均较MC组显著上调(P
AimTo evaluate the effects of quercetin to improve ischemia/reperfusion-induced cardiomyocyte apoptosis in vitro and in vivo study.MethodsThe cells were divided into five groups: model control (MC) group was ischemia/reperfusion (I/R) model group; DL group was treated with 25mL/L quercetin based on MC group; DM group was treated with 50ml/L quercetin based on MC group; DH group was treated with 100mL/L quercetin based on MC group; Meto group was treated with metoprolol based on MC group. In the invivo study, the rats were divided into five groups: MC group was I/R model group; DL group was treated with 25mg/kg quercetin; DM group was treated with 50mg/kg quercetin; DM group was treated with 100mg/kg quercetin; Meto group was treated with Meto as positive drug.ResultsThe cell apoptosis rates of quercetin treated groups (DL, DM, and DH groups) were significantly suppressed compared with the MC group. The silent information regulatory factor 1 (SIRT1), peroxisome proliferators-activated receptor- coactivator-1 (PGC-1), and Bcl-2 proteins expression of quercetin treated were significantly upregulation compared with MC group (P