In vitro and ex vivo anti-inflammatory activity of quercetin in healthy volunteers
In vitro and ex vivo anti-inflammatory activity of quercetin in healthy volunteers
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DOI：
10.1016/j.nut.2008.03.023
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发表时间：
2008-07-01
期刊：
影响因子：
4.4
通讯作者：
Haenen, Guido R. M. M.
中科院分区：
文献类型：
--
作者：
Boots, Agnes W.;Wilms, Lonneke C.;Haenen, Guido R. M. M.
目的：槲皮素是一种常见的黄酮类化合物，也是一种抗氧化剂。本研究探讨了生理上可达到的槲皮素浓度可能的抗炎作用。方法：槲皮素的作用进行了体外试验，即，加入到试管中的血液中，以及离体和体内，即，结果：槲皮素剂量依赖性地抑制体外脂多糖诱导的健康志愿者血液中肿瘤坏死因子-a的产生。在1 μ M的浓度下，槲皮素引起23%的减少。体外脂多糖诱导的白细胞介素-10的生产仍然不受槲皮素。4周槲皮素干预导致血浆槲皮素浓度显着增加。补充也增加了总血浆抗氧化状态，但不影响谷胱甘肽，维生素C和尿酸血浆浓度。基础和体外脂多糖诱导的肿瘤坏死因子-a水平没有改变的干预。结论：本研究表明，槲皮素。在健康志愿者的血液中，增加体内抗氧化能力，并在体外显示抗炎作用，但在体内或离体不显示抗炎作用。这种缺乏效果可能是由于其基线时的低细胞因子和高抗氧化剂水平，表明既不存在炎症也不存在氧化应激。只有在炎症和氧化应激水平升高的人群中，例如，对于病理学与这两个过程相关的疾病患者，补充抗氧化剂可能是有效的。(C)2008年爱思唯尔公司All rights reserved.
Objective: Quercetin, a commonly occurring flavonoid and well known antioxidant, has been suggested to possess other beneficial activities. The present study investigated the possible anti-inflammatory effects of physiologically attainable quercetin concentrations.Methods: The effects of quercetin were tested in vitro, i.e., added to blood in the test tube, and ex vivo and in vivo, i.e., in blood taken after 4 wk of administration of quercetin in an intervention study.Results: Quercetin dose-dependently inhibited in vitro lipopolysaccharide-induced tumor necrosis factor-a production in the blood of healthy volunteers. At a concentration of 1 mu M, quercetin caused a 23% reduction. The in vitro lipopolysaccharide-induced interleukin-10 production remained unaffected by quercetin. A 4-wk quercetin intervention resulted in a significant increase in plasma quercetin concentration. The supplementation also increased total plasma antioxidant status but did not affect glutathione, vitamin C, and Uric acid plasma concentrations. Basal and ex vivo lipopolysaccharide-induced tumor necrosis factor-a levels were not altered by the intervention.Conclusion: The present study shows that quercetin. increases antioxidant capacity in vivo and displays anti-inflammatory effects in vitro, but not in vivo or ex vivo, in the blood of healthy volunteers. This lack of effect is probably due to their low cytokine and high antioxidant levels at baseline, indicating that neither inflammation nor oxidative stress is present. Only in people with increased levels of inflammation and oxidative stress, e.g., patients with a disease of which the pathology is associated with these two processes, might antioxidant supplementation be fruitful. (C) 2008 Elsevier Inc. All rights reserved.