Establishing immune scoring model based on combination of the number, function, and phenotype of lymphocytes
Establishing immune scoring model based on combination of the number, function, and phenotype of lymphocytes
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DOI：
10.18632/aging.103208
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发表时间：
2020-05-31
期刊：
影响因子：
5.2
通讯作者：
Sun, Ziyong
中科院分区：
文献类型：
--
作者：
Tang, Guoxing;Yuan, Xu;Sun, Ziyong
背景资料：结果：淋巴细胞的数量、功能和表型中的大多数参数与年龄相关。在四个年龄组（儿童、青少年、成人和老年人）中确定了这些参数的参考范围。CD 4（+）T细胞、CD 8（+）T细胞、B细胞与年龄呈负相关，NK细胞与年龄无相关性。而CD 4（+）T细胞、CD 8（+）T细胞和NK细胞功能与年龄呈正相关。随着年龄的增长，T细胞表面CD 28的表达逐渐下降，并与其功能呈负相关。T细胞表面HLA-DR和CD 45 RO的表达则相反。采用8个参数（CD 4（+）T细胞数×功能、CD 28（+）CD 4（+）T细胞数、HLA-DR（+）CD 4（+）T细胞数、CD 45 RO（+）CD 4（+）T细胞数、CD 8（+）T细胞数×功能、CD 28（+）CD 8（+）T细胞数、HLA-DR（+）CD 8（+）T细胞数、NK细胞数量x功能）从淋巴细胞数量、功能和表型的结果。结论：结合淋巴细胞数量、功能和表型建立的免疫评分模型在定量评价宿主免疫功能方面具有潜在的应用价值。同时测定CD 4（+）T细胞、CD 8（+）T细胞和NK细胞的数量、功能和表型。
Background: Quantitatively assessing host immunity remains a challenge in clinical practice.Results: Most parameters in lymphocyte number, function and phenotype were correlated with age. The reference ranges of these parameters were established in four age groups (children, adolescents, adults, and elders). The numbers of CD4(+) T cells, CD8(+) T cells, B cells, but not NK cells, were negatively correlated with age. However, the function of CD4(+) T cells, CD8(+) T cells and NK cells was positively correlated with age. The expression of CD28 on T cells gradually decreased with increasing age and was negatively correlated with their function. An opposite phenomenon was observed in the expressions of HLA-DR and CD45RO on T cells. An immune scoring model was established by using 8 parameters (CD4(+) T cell number x function, CD28(+)CD4(+) T cell number, HLA-DR(+)CD4(+) T cell number, CD45RO(+)CD4(+) T cell number, CD8(+) T cell number x function, CD28(+)CD8(+) T cell number, HLA-DR(+)CD8(+) T cell number, NK cell number x function) from the results of lymphocyte number, function, and phenotype. This immune scoring model showed sensitivities of 70% and 71.4% in determining hyper-immune and hypo-immune status, respectively.Conclusions: An immune scoring model based on combination of lymphocyte number, function, and phenotype shows potential value in quantitatively assessing host immunity.Methods: 261 healthy individuals aged 1 to 82 years were recruited from Tongji Hospital. The number, function, and phenotype of CD4(+) T cells, CD8(+) T cells and NK cells were simultaneously determined.