Stratum corneum lipidomics analysis reveals altered ceramide profile in atopic dermatitis patients across body sites with correlated changes in skin microbiome
Stratum corneum lipidomics analysis reveals altered ceramide profile in atopic dermatitis patients across body sites with correlated changes in skin microbiome
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DOI：
10.1111/exd.14185
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发表时间：
2020-09-17
影响因子：
3.6
通讯作者：
Weidinger, Stephan
中科院分区：
文献类型：
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作者：
Emmert, Hila;Baurecht, Hansjoerg;Weidinger, Stephan
背景 特应性皮炎 (AD) 是由表皮屏障功能失调和皮肤免疫失调之间的相互作用驱动的。作为复杂皮肤屏障功能障碍的一部分，脂质组织和微生物组组成的异常已被描述。我们着手系统地研究 AD 患者和健康志愿者三个不同身体部位的角质层脂质组、皮肤微生物组和皮肤生理参数的组成。方法 我们使用 Metabolon 质谱平台分析了取自 10 名 AD 成人和 10 名健康志愿者的不同身体部位的胶带，这些胶带与 361 种皮肤脂质的 FLG 突变状态相匹配。所有先证者均可获得 16S rRNA 数据。结果我们的研究表明，不同身体部位以及 AD 患者和健康个体之间的脂质成分存在显着差异。与健康志愿者相比，AD 患者中的神经酰胺种类 NS 显着更高，并且与没有 aFLG 突变的 AD 患者相比，有 aFLG 突变的 AD 患者中的神经酰胺种类 NS 也更高。皮肤脂质变化与微生物组的相关性分析表明，AD 中葡萄球菌的定植与神经酰胺亚种 AS、ADS、NS 和 NDS 呈正相关。结论 这是第一项揭示 AD 中位点特异性脂质改变及其与皮肤微生物组相关性的研究。
Background Atopic dermatitis (AD) is driven by the interplay between a dysfunctional epidermal barrier and a skewed cutaneous immune dysregulation. As part of the complex skin barrier dysfunction, abnormalities in lipid organization and microbiome composition have been described. We set out to systematically investigate the composition of the stratum corneum lipidome, skin microbiome and skin physiology parameters at three different body sites in patients with AD and healthy volunteers. Methods We analysed tape strips from different body areas obtained from 10 adults with AD and 10 healthy volunteers matched forFLGmutation status for 361 skin lipid species using the Metabolon mass spectrometry platform. 16S rRNA data were available from all probands. Results Our study showed that the lipid composition differs significantly between body sites and between AD patients and healthy individuals. Ceramide species NS was significantly higher in AD patients compared to healthy volunteers and was also higher in AD patients with aFLGmutation compared to AD patients without aFLGmutation. The correlation analysis of skin lipid alterations with the microbiome showed that Staphylococcus colonization in AD is positively correlated with ceramide subspecies AS, ADS, NS and NDS. Conclusion This is the first study to reveal site-specific lipid alterations and correlations with the skin microbiome in AD.