Circadian programming of the ellipsoid body sleep homeostat in Drosophila.
Circadian programming of the ellipsoid body sleep homeostat in Drosophila.
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DOI：
10.7554/elife.74327
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发表时间：
2022-06-23
期刊：
影响因子：
7.7
通讯作者：
Allada, Ravi
中科院分区：
文献类型：
--
作者：
Andreani, Tomas;Rosensweig, Clark;Sisobhan, Shiju;Ogunlana, Emmanuel;Kath, William;Allada, Ravi
稳态和昼夜节律过程协作，以适当地定时和巩固睡眠和觉醒。为了了解这些过程是如何整合的，我们在果蝇中安排了一天中不同时间的短暂睡眠剥夺，发现早晨的反弹比晚上高。这些效应取决于离散的早晨和晚上的时钟神经元，而与它们在昼夜运动活动中的作用无关。在R5椭球体睡眠稳态，我们确定了活动依赖性和突触前基因表达以及突触前蛋白BRUCHPILOT与时钟电路调节一致的早晨表达升高。这些神经元也显示出钙水平升高，以响应早上的睡眠不足，但不是晚上与观察到的时间依赖性睡眠反弹一致。这些研究揭示了离散的生物钟神经元对稳态睡眠中心进行编程的电路和分子机制。
Homeostatic and circadian processes collaborate to appropriately time and consolidate sleep and wake. To understand how these processes are integrated, we scheduled brief sleep deprivation at different times of day in Drosophila and find elevated morning rebound compared to evening. These effects depend on discrete morning and evening clock neurons, independent of their roles in circadian locomotor activity. In the R5 ellipsoid body sleep homeostat, we identified elevated morning expression of activity dependent and presynaptic gene expression as well as the presynaptic protein BRUCHPILOT consistent with regulation by clock circuits. These neurons also display elevated calcium levels in response to sleep loss in the morning, but not the evening consistent with the observed time-dependent sleep rebound. These studies reveal the circuit and molecular mechanisms by which discrete circadian clock neurons program a homeostatic sleep center.