Inhibiti on of T-Cell-Mediated and Infection-Ind uced Periodontal Bone Resorption by TAC E Blockade
Inhibiti on of T-Cell-Mediated and Infection-Ind uced Periodontal Bone Resorption by TAC E Blockade
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TAC E 阻断对 T 细胞介导和感染诱导的牙周骨吸收的抑制
DOI：
10.1007/978-4-431-54070-0_45
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
M
中科院分区：
文献类型：
--
作者：
Kanzaki;H.; Han;X.; Asami;Y.; Suzu ki;M.; Kawai;T.; Taubman;M
牙周炎的宿主免疫反应促进骨吸收，浸润淋巴细胞表达丰富的RANKL，这是破骨细胞形成的必要因子。由于RANKL最初是作为一种膜结合蛋白产生的，因此破骨细胞的形成可能需要细胞间的接触。然而，淋巴细胞和活跃破骨细胞之间的距离通常被观察到。这一观察推测活化淋巴细胞释放的细胞因子可能诱导牙周炎的破骨细胞生成。先前，我们报道了淋巴细胞中TNF-α转换酶（TACE）裂解RANKL和TNF-α可能在人类牙周炎中发挥作用。在此，我们进一步研究了TACE在两种实验性大鼠牙周炎模型中的功能：(1)聚合杆菌放线菌-反应性t细胞过继转移和(2)牙龈卟啉单胞菌感染。在牙龈内注射抗TACE抗体，探讨TACE在骨破坏中的作用。我们的数据表明，TACE阻断可以抑制牙槽骨破坏。TACE可能是改善牙周炎的潜在治疗靶点。
Host immune responses trigger promotion of bone resorption in periodontitis, and infiltrated lymphocytes express abundant RANKL, the essential factor for osteoclastogenesis. Since RANKL is initially produced as a membrane-bound protein, cell-to-cell contact can be required for osteoclastogenesis. However, distance between lymphocytes and active osteoclasts is generally observed. This observation presume released cytokine(s) from activated lymphocytes might induce osteoclastogenesis in periodontitis.Previously, we reported that cleavage of RANKL and TNF-α by TNF-α converting enzyme (TACE) from lymphocytes can play a role in human periodontitis. Herein, we further examined function of TACE in two experimental periodontitis models in rats: (1) Aggregatibacter actinomycetemcomitans-reactive T-cell adoptive transfer and (2)Porphyromonas gingivalisinfection. Anti-TACE antibody was injected into the gingivae to probe the role of TACE in bone destruction. Our data suggested that TACE blockade could inhibit alveolar bone destruction. TACE might be a potentially therapeutic target for periodontitis amelioration.