Orexin-A in the human brain and tumor tissues of ganglioneuroblastoma and neuroblastoma
Orexin-A in the human brain and tumor tissues of ganglioneuroblastoma and neuroblastoma
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DOI：
10.1016/s0196-9781(00)00184-4
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发表时间：
2000-04-01
期刊：
影响因子：
3
通讯作者：
Mouri, T
中科院分区：
文献类型：
--
作者：
Arihara, Z;Takahashi, K;Mouri, T
本文用放射免疫分析法研究了食欲素A（orexin-A）免疫反应物在人脑和垂体的区域分布，以及在嗜铬细胞瘤、神经节细胞瘤和神经母细胞瘤肿瘤组织中的存在。采用逆转录聚合酶链反应（RT-PCR）方法检测食欲素mRNA的表达。食欲素A样免疫反应在人脑的每个区域，但没有在垂体中检测到。在人脑中，食欲素A样免疫反应性的最高浓度见于下丘脑（17.8 +/- 4.3 pmol/g湿重，平均值+/- SEM，n = 7），其次是丘脑、延髓和笔。Orexin-A在神经节细胞瘤和神经母细胞瘤中呈阳性表达，而在嗜铬细胞瘤中未见阳性表达。反相高效液相色谱分析人脑提取物和神经母细胞瘤提取物中的食欲素A样免疫反应性显示单一的免疫反应性峰，其洗脱位置与合成的人食欲素A相同。食欲素mRNA在下丘脑和神经节神经母细胞瘤和神经母细胞瘤的肿瘤组织中表达。这些发现表明食欲素-A在下丘脑中产生，并通过轴突运输到大脑的各个区域。此外，这项研究首次显示了神经节神经母细胞瘤和神经母细胞瘤产生食欲素A。(C)2000 Elsevier Science Inc. All rights reserved.
Regional distribution of orexin-A-like immunoreactivity in the human brain and pituitary, and the presence of orexin-A-like immunoreactivity in the tumor tissues of pheochromocytomas, ganglioneuroblastomas and neuroblastomas were studied by radioimmunoassay. Expression of orexin mRNA was studied by reverse transcriptase polymerase chain reaction (PCR) method. Orexin-A-like immunoreactivity was detected in every region of human brain, but not in the pituitary. The highest concentration of orexin-A-like immunoreactivity in the human brain was found in hypothalamus (17.8 +/- 4.3 pmol/g wet weight, mean +/- SEM, n = 7), followed by thalamus, medulla oblongata, and pens. Orexin-A-like immunoreactivity was detected in the tumor tissues of ganglioneuroblastoma and neuroblastoma, but not in the tumor tissues of pheochromocytoma. Reverse phase high performance liquid chromatographic analyses of the orexin-A-like immunoreactivity in the human brain extracts and neuroblastoma extracts showed a single immunoreactive peak, which was eluted in an identical position to synthetic human orexin-A, Orexin mRNA was expressed in the hypothalamus and in the tumor tissues of ganglioneuroblastoma and neuroblastoma. These findings suggest that orexin-A is produced in the hypothalamus and transported to various brain regions via axons. In addition, this study has shown for the first time the production of orexin-A by ganglioneuroblastomas and neuroblastomas. (C) 2000 Elsevier Science Inc. All rights reserved.