Curcumin treatment enhances islet recovery by induction of heat shock response proteins, Hsp70 and heme oxygenase-1, during cryopreservation
Curcumin treatment enhances islet recovery by induction of heat shock response proteins, Hsp70 and heme oxygenase-1, during cryopreservation
复制标题
DOI：
10.1016/j.lfs.2007.10.026
复制
发表时间：
2008-01-16
期刊：
影响因子：
6.1
通讯作者：
Bhonde, Ramesh R.
中科院分区：
文献类型：
--
作者：
Kanitkar, Meghana;Bhonde, Ramesh R.
在糖尿病治疗中，胰岛冷冻保存后恢复有限影响移植物的存活和移植效率。由于姜黄素是一种有效的抗氧化/自由基清除化合物，可以保护胰岛免受β细胞毒素的侵害，我们假设在冷冻保存或解冻后培养或两者同时加入姜黄素可以拯救胰岛免受冷冻损伤。为了研究姜黄素包合对胰岛恢复的影响，采用胶原酶消化法分离小鼠胰岛，培养48 h，用二甲亚砜作为冷冻保护剂（含或不含姜黄素10 μ M）冷冻保存，然后缓慢冷却至-40℃，然后将其浸泡在液氮中7天。通过蔗糖梯度快速解冻和解冻后24小时的培养-存在或不存在姜黄素(10 μ M) -测定胰岛活力和功能。姜黄素处理组的胰岛恢复明显高于不含姜黄素的胰岛冷冻保存组。姜黄素冷冻保存的胰岛比没有姜黄素冷冻保存的胰岛更完整，形态学更好。与没有姜黄素的胰岛相比，姜黄素处理的胰岛也显示出明显的ROS生成抑制。姜黄素冷冻保存的胰岛的葡萄糖反应性和胰岛素分泌与未冷冻保存的胰岛相同。在姜黄素冷冻保存的胰岛中观察到hsp70和HO-1水平升高，这可能与姜黄素诱导的胰岛拯救有关。因此，我们得出结论，在低温保存培养基中加入姜黄素可以抑制ROS的产生和相应的胰岛损伤和功能障碍。(c) 2007爱思唯尔公司版权所有。
Limited recovery of islets post-cryopreservation influences graft survival and transplantation efficiency during diabetes treatment. As curcumin, a potent antioxidant/radical scavenging compound, protects islets against beta cell toxins, we hypothesized that inclusion of curcumin during cryopreservation or during post-thaw culture or both may rescue islets from cryoinjury. To test the effect of curcumin inclusion on islet recovery murine islets were isolated by the collagenase digestion, cultured for 48 h, cryopreserved using dimethylsulphoxide as cryoprotectant - with or without curcumin (10 mu M) - and then slow cooled to -40 degrees C before immersing them in liquid nitrogen for 7 days. Following rapid thawing with sucrose gradient and 24 h post-thaw culture - in presence or absence of curcumin (10 mu M) - islet viability and functionality were determined. Islet recovery in curcumin treated groups was significantly higher than in groups where islets were cryopreserved without curcumin. Islets cryopreserved with curcumin also showed more intact islets as well as better morphology as compared to islets cryopreserved without curcumin. Curcumin treated islets also showed significant inhibition of ROS generation as compared to islets cryopreserved without curcumin. Glucose responsiveness and insulin secretion in islets cryopreserved with curcumin was equal to that of the freshly isolated islets as against islets cryopreserved without curcumin. Elevated level of Hsp 70 and HO-1 were observed in islets cryopreserved with curcumin and may contribute to curcumin-induced islet rescue. Hence, we conclude that inclusion of curcumin into cryopreservation medium inhibits ROS generation and corresponding islet damage and dysfunction. (c) 2007 Elsevier Inc. All rights reserved.