CLONING, SEQUENCE, AND EXPRESSION OF THE LYSOSTAPHIN GENE FROM STAPHYLOCOCCUS-SIMULANS
CLONING, SEQUENCE, AND EXPRESSION OF THE LYSOSTAPHIN GENE FROM STAPHYLOCOCCUS-SIMULANS
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DOI：
10.1073/pnas.84.5.1127
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发表时间：
1987-03-01
影响因子：
11.1
通讯作者：
NOVICK, RP
中科院分区：
文献类型：
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作者：
RECSEI, PA;GRUSS, AD;NOVICK, RP
一个1.5-内切酶对的DNA片段，包含溶葡萄球菌酶基因从模拟葡萄球菌及其侧翼序列已被克隆和完全测序。该基因编码一个Mr为42，000的前酶原。前酶原的NH 2-末端序列由信号肽和随后的13个氨基酸序列的7个串联重复组成。溶葡萄球菌酶原转化为成熟酶发生在S. simulans，并涉及去除含有串联重复序列的酶原的NH 2-末端部分。重复序列的高度同源性表明，它们是由39个碱基对的DNA序列复制而产生的。In S.在simulans中，溶葡萄球菌酶基因存在于一个大的β-内酰胺酶质粒。
A 1.5-kilobase-pair fragment of DNA that contains the lysostaphin gene from Staphylococcus simulans and its flanking sequences has been cloned and completely sequenced. The gene encodes a preproenzyme of Mr 42,000. The NH2-terminal sequence of the preproenzyme is composed of a signal peptide followed by seven tandem repeats of a 13-amino acid sequence. Conversion of prolysostaphin to the mature enzyme occurs extracellularly in cultures of S. simulans and involves removal of the NH2-terminal portion of the proenzyme that contains the tandem repeats. The high degree of homology of the repeats suggests that they have arisen by duplication of a 39-base-pair sequence of DNA. In S. simulans, the lysostaphin gene is present on a large .beta.-lactamase plasmid.