Ion mobility mass spectrometry of peptide, protein, and protein complex ions using a radio-frequency confining drift cell
Ion mobility mass spectrometry of peptide, protein, and protein complex ions using a radio-frequency confining drift cell
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DOI：
10.1039/c5an02107c
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发表时间：
2016-01-01
期刊：
影响因子：
4.2
通讯作者：
Bush, Matthew F.
中科院分区：
文献类型：
--
作者：
Allen, Samuel J.;Giles, Kevin;Bush, Matthew F.
离子迁移率质谱实验能够表征生物分子和组装体的质量、组装和形状。在这里，一个新的射频限制漂移池的特点和用于测量的迁移率的肽，蛋白质和蛋白质复合物离子。新的漂移池取代了沃茨Synapt G2 HDMS中的行波离子迁移池。在原始仪器控制软件的背景下，提出了使用该实验装置操作漂移池和确定碰撞截面值的方法。碰撞截面为349阳离子和阴离子的报告，其中155个是以前没有使用离子迁移率的特点的离子。剩余离子的值类似于使用先前的射频限制漂移室和没有径向限制的漂移管确定的那些。使用该装置下2托的氦气和优化的漂移电压，变性和类天然离子表现出的平均表观分辨率分别为14.2和16.5，分别。对于具有高迁移率的离子，其质量也较低，表观分辨能力受到来自离子门控的贡献的限制。相比之下，到达时间分布的低迁移率，原生类离子没有得到很好的解释，只使用离子门控和扩散的贡献。对于这些物种，到达时间分布的宽度是最一致的存在下，在气相中的多个结构。
Ion mobility mass spectrometry experiments enable the characterization of mass, assembly, and shape of biological molecules and assemblies. Here, a new radio-frequency confining drift cell is characterized and used to measure the mobilities of peptide, protein, and protein complex ions. The new drift cell replaced the traveling-wave ion mobility cell in a Waters Synapt G2 HDMS. Methods for operating the drift cell and determining collision cross section values using this experimental set up are presented within the context of the original instrument control software. Collision cross sections for 349 cations and anions are reported, 155 of which are for ions that have not been characterized previously using ion mobility. The values for the remaining ions are similar to those determined using a previous radio-frequency confining drift cell and drift tubes without radial confinement. Using this device under 2 Torr of helium gas and an optimized drift voltage, denatured and native-like ions exhibited average apparent resolving powers of 14.2 and 16.5, respectively. For ions with high mobility, which are also low in mass, the apparent resolving power is limited by contributions from ion gating. In contrast, the arrival-time distributions of low-mobility, native-like ions are not well explained using only contributions from ion gating and diffusion. For those species, the widths of arrival-time distributions are most consistent with the presence of multiple structures in the gas phase.