Bacterial community on ectomycorrhizal roots of Laccaria laccata in a chestnut plantation
Bacterial community on ectomycorrhizal roots of Laccaria laccata in a chestnut plantation
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DOI：
10.1016/j.myc.2018.08.002
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发表时间：
2019
期刊：
影响因子：
1.4
通讯作者：
Keisuke Obase
中科院分区：
文献类型：
--
作者：
Keisuke Obase
采用培养和克隆的方法研究了漆蜡蘑外生菌根根上的细菌群落。从五个地点采集了一个土芯，其中L.在板栗种植园中发生了虫蛀。分别取10个和50个L.从每个土芯收集漆细胞，并进行细菌分离和克隆。223个分离株被划分为35个分子操作分类单位（MOTU）。大多数MOTU是罕见的，而一个MOTU根瘤菌和慢生根瘤菌是常见的整个采样位置。在基于克隆的方法中，检测到77个MOTU。这些MOTU的大多数是罕见的，而一个MOTU每一个慢生根瘤菌和一个分类群的γ-变形菌是常见的整个采样位置。这些结果表明，慢生根瘤菌是L.板栗种植园中的漆叶。© 2018日本真菌学会。Laccaria laccata（Hydnangiaceae，Agaricales，Basidiomycota）是一种外生菌根真菌，常见于北方至温带森林的壳斗科、桦木科、杨柳科和松科植物的根部（Kropp & Mueller，1999）。这种真菌的生态特征表明，它是一个先锋物种，经常发现在干扰地区与年轻的立场，如火山沙漠（奈良，Nakaya，和Hogetsu，2003年）和种植园（例如，梅森，最后，Pelham，和Ingleby，1982年）。因此，L.漆孢菌被认为是一种有前途的外生菌根真菌，可用作在苗圃条件下促进幼苗生长的生物资源，因此已被广泛用于应用研究（例如，Sinclair，Sylvia，& Larsen，1982）。另一方面，在实验条件下，真菌可以容易地操作，并且在接种到相容的宿主幼苗上（例如，Molina，1982）或甚至在无宿主植物的无菌条件下（Davis & Jong，1976），也可以相对容易地诱导子实体的产生。因此，该真菌也可用作研究真菌本身生物学以及与其他生物体的生物相互作用的基础研究的模式物种，如L.已经被广泛研究用于理解作为模式物种的真菌生物学和生态学（例如，Martin等人，2008年）。
This study investigated the bacterial communities present on the ectomycorrhizal roots of Laccaria laccata using culture-and cloning-based methods. One soil core was collected from five locations where sporocarps of L. laccata occurred in a chestnut plantation. A total of 10 and 50 ectomycorrhizal root tips of L. laccata were collected from each soil core and subjected to bacterial isolation and cloning. In total, 223 isolates were classified into 35 molecular operational taxonomic units (MOTUs). The majority of MOTUs were infrequent, whereas one MOTU each of Rhizobium and Bradyrhizobium were common across the sampling locations. In the cloning-based method, 77 MOTUs were detected. The majority of these MOTUs were infrequent, whereas one MOTU each of Bradyrhizobium and one taxon of Gammaproteobacteria was common across the sampling locations. These results indicate that Bradyrhizobium is a common member of bacteria present on the ectomycorrhizal roots of L. laccata in the chestnut plantation.© 2018 The Mycological Society of Japan. Published by Elsevier BV All rights reserved.Laccaria laccata (Hydnangiaceae, Agaricales, Basidiomycota) is an ectomycorrhizal (EcM) fungus that is commonly associated with the roots of plants belonging to the families Fagaceae, Betulaceae, Salicaceae, and Pinaceae in boreal to temperate forests (Kropp & Mueller, 1999). The ecological characteristics of this fungus indicate that it is a pioneer species and often found in disturbed areas with young stands, such as volcanic deserts (Nara, Nakaya, & Hogetsu, 2003) and plantations (eg, Mason, Last, Pelham, & Ingleby, 1982). Therefore, L. laccata has been regarded as one of the promising EcM fungi that can be used as a bioresource for promoting seedling growth in nursery conditions and thus has been used extensively in applied research (eg, Sinclair, Sylvia, & Larsen, 1982). On the other hand, the fungus can be easily manipulated under experimental conditions and the production of sporocarps can also be relatively easily induced after inoculation onto compatible host seedlings (eg, Molina, 1982) or even under axenic conditions without host plants (Davis & Jong, 1976). Hence, the fungus can also be used as a model species for basic research investigating the biology of the fungus itself and the biological interactions with other organisms, as L. bicolor that has been extensively studied for understanding fungal biology and ecology as a model species (eg, Martin et al., 2008).