Proteomics study of serum exosomes in Kawasaki disease patients with coronary artery aneurysms.
Proteomics study of serum exosomes in Kawasaki disease patients with coronary artery aneurysms.
复制标题
川崎病冠状动脉瘤患者血清外泌体蛋白质组学研究
DOI：
10.5603/cj.a2018.0032
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发表时间：
2019
影响因子：
2.9
通讯作者：
Zhang L
中科院分区：
文献类型：
--
作者：
Xie XF;Chu HJ;Xu YF;Hua L;Wang ZP;Huang P;Jia HL;Zhang L
背景：研究川崎病(KD)所致冠状动脉瘤(CAA)患者血清外显体的蛋白质谱。方法采用双向凝胶电泳法(2-DE)对川崎病(KD)所致CAA患儿和健康儿童血清外显体蛋白质进行鉴定。用基质辅助激光解吸/电离飞行时间/飞行时间质谱仪(MALDI-TOF/TOF MS)分析鉴定差异表达的蛋白质。结果从CAA患儿血清Exosome中鉴定出32个差异表达蛋白(18个上调，14个下调)，并与正常对照组进行比较。用Western blotting验证TN、RBP4、LRG1和APOA4四种蛋白的表达水平。分类分析和蛋白质-蛋白质网络分析表明，它们与多种功能基团相关，包括宿主免疫反应、炎症、凋亡过程、发育过程和生物黏附过程。结论本研究建立了KD所致CAA患儿血清外切体的蛋白质组学图谱，为KD所致CAA的发病机制提供了新的认识。
Background To study the protein profile of the serum exosomes of patients with coronary artery aneurysms (CAA) caused by Kawasaki disease (KD). Methods Two-dimensional electrophoresis (2-DE) was used to identify proteins from the exosomes of serum obtained from children with CAA caused by KD, as well as healthy controls. Differentially expressed proteins were identified using matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF MS) analysis. Results Thirty two differentially expressed proteins were identified (18 up-regulated and 14 downregulated) from serum exosomes of children with CAA and were compared to healthy controls. The expression levels of 4 proteins (TN, RBP4, LRG1, and APOA4) were validated using Western blotting. Classification analysis and protein–protein network analysis showed that they are associated with multiple functional groups, including host immune response, inflammation, apoptotic process, developmental process, and biological adhesion process. Conclusions These findings establish a comprehensive proteomic profile of serum exosomes from children with CAA caused by KD, and provide additional insights into the mechanisms of CAA caused by KD.