Transcriptomic view of survival during early seedling growth of the extremophyte Haloxylon ammodendron
Transcriptomic view of survival during early seedling growth of the extremophyte Haloxylon ammodendron
复制标题
极端植物梭梭幼苗早期生长存活的转录组学研究
DOI：
10.1016/j.plaphy.2018.09.024
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发表时间：
2018-11-01
影响因子：
6.5
通讯作者：
Qiu, Quan-Sheng
中科院分区：
文献类型：
--
作者：
Fan, Ligang;Wang, Guannan;Qiu, Quan-Sheng
在极端环境中建立幼苗需要一个综合的基因组和生理反应，以生存多种非生物胁迫。梭梭是一种能够在温带荒漠沙丘定居的先锋植物。我们研究了H.梭梭幼苗早期的水分亏缺胁迫。我们发现，不仅干旱响应基因，但多个基因的途径与盐，渗透，冷，紫外线和高光应力诱导，这表明改变了调节应激反应系统。此外，H.通过下调在易感植物中病原体进入期间通常上调的基因，红木表现出增强的生物胁迫耐受性。通过比较H.与苋科中6个相近的转录组相比，我们在梭梭中检测到了丰富的基础水平转录本。对非生物胁迫和病原体表现出预适应性的梭梭。我们发现转录本一般维持在低水平，一些只在非生物胁迫下诱导的压力敏感模型，拟南芥在基础条件下，在苋科转录组，包括H。梭梭H.梭梭表现出协调的基因表达，调节胁迫耐受性和幼苗发育资源分配，以支持在沙丘为主的温带沙漠环境中抵抗多种胁迫的生存。
Seedling establishment in an extreme environment requires an integrated genomic and physiological response to survive multiple abiotic stresses. The extremophyte, Haloxylon ammodendron is a pioneer species capable of colonizing temperate desert sand dunes. We investigated the induced and basal transcriptomes in H. ammodendron under water-deficit stress during early seedling establishment. We find that not only drought-responsive genes, but multiple genes in pathways associated with salt, osmotic, cold, UV, and high-light stresses were induced, suggesting an altered regulatory stress response system. Additionally, H. atrunodendron exhibited enhanced biotic stress tolerance by down-regulation of genes that were generally up-regulated during pathogen entry in susceptible plants. By comparing the H. ammodendron basal transcriptome to six closely related transcriptomes in Amaranthaceae, we detected enriched basal level transcripts in H. ammodendron that shows preadaptation to abiotic stress and pathogens. We found transcripts that were generally maintained at low levels and some induced only under abiotic stress in the stress-sensitive model, Arabidopsis thaliana to be highly expressed under basal conditions in the Amaranthaceae transcriptomes including H. ammodendron. H. ammodendron shows coordinated expression of genes that regulate stress tolerance and seedling development resource allocation to support survival against multiple stresses in a sand dune dominated temperate desert environment.