Schizosaccharomyces pombe Ppr10 is required for mitochondrial translation
Schizosaccharomyces pombe Ppr10 is required for mitochondrial translation
复制标题
粟酒裂殖酵母 Ppr10 是线粒体翻译所必需的
DOI：
10.1093/femsle/fnaa170
复制
发表时间：
2020
影响因子：
2.1
通讯作者：
Huang Ying
中科院分区：
文献类型：
--
作者：
Liu Zecheng;Li Yan;Xie Wanqiu;Huang Ying
线粒体基因组编码氧化磷酸化（OXPHOS）系统的关键组分，其表达对线粒体功能至关重要。我们以前已经表明，缺失的theSchizoschiomycespombe ppr10编码的五肽重复蛋白严重降低了成熟水平的内含子含有线粒体转录scox 1和cob1，并严重损害线粒体翻译。在这项研究中，我们研究了细胞中Cox1和Cob1蛋白水平降低是由于cox1和Cob1mRNA水平降低的可能性。我们发现，在线粒体无内含子背景下，ppr10的缺失并不影响maturecox1和cob1mRNA的水平。然而，Cox1和Cob1蛋白质的合成仍然受到严重影响的缺失ppr10在线粒体内含子的背景。与此相一致，我们发现线粒体内含子的缺失不能挽救Δ ppr10细胞的呼吸生长缺陷。我们的研究结果表明，Ppr10是不需要的稳定cox 1和cob1的mRNA，并提供了进一步的支持的想法，Ppr10在线粒体翻译中起着关键作用。
The mitochondrial genome encodes key components of the oxidative phosphorylation (OXPHOS) system, whose expression is essential for mitochondrial functions. We have previously shown that deletion of theSchizosaccharomyces pombe ppr10encoding a pentatricopeptide repeat protein severely reduces the mature levels of intron-containing mitochondrial transcriptscox1andcob1, and severely impairs mitochondrial translation. In this study, we examined the possibility that the reduced levels of Cox1 and Cob1 proteins in cells were due to lowered levels ofcox1andcob1mRNAs. We found that deletion ofppr10did not affect the levels of maturecox1andcob1mRNAs in a mitochondrial intronless background. However, synthesis of Cox1 and Cob1 proteins were still severely affected by deletion ofppr10in a mitochondrial intronless background. Consistent with this, we found that deletion of mitochondrial introns could not rescue the respiratory growth defect of Δppr10cells. Our results reveal that Ppr10 is not required for the stability ofcox1andcob1mRNAs, and provide further support for the idea that Ppr10 plays a critical role in mitochondrial translation.