An Autographa californica nucleopolyhedrovirus-encoded microRNA, AcMNPV-miR-4, downregulates the expression of host gene alg-2.
An Autographa californica nucleopolyhedrovirus-encoded microRNA, AcMNPV-miR-4, downregulates the expression of host gene alg-2.
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DOI：
10.1099/jgv.0.001769
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发表时间：
2022-07
期刊：
影响因子：
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通讯作者：
Jin Zhao;Tingkai Teng;Jinwen Wang
中科院分区：
文献类型：
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作者：
Jin Zhao;Tingkai Teng;Jinwen Wang
苜蓿银纹夜蛾核型多角体病毒（Autographa california multiple nucleopolyhedrovirus，AcMNPV）编码的调节病毒基因以实现感染的microRNA（miRNAs）先前已被报道。在此，我们报道了另一种AcMNPV编码的miRNA，AcMNPV-miR-4（Ac-miR-4），其下调宿主基因，即阿尔茨海默病相关基因（alg-2）。通过双荧光素酶报告基因测定验证了这种调节。评估Ac-miR-4对病毒感染的影响。结果显示，当给予过量的Ac-miR-4时，感染性出芽病毒体（BV）的产生减少，并且包埋到多角体中的封闭衍生病毒体（ODV）被延迟。所有这些发现表明Ac-miR-4延长细胞寿命并在相对早期阶段降低病毒毒力，但在非常晚期阶段增加ODV。这一发现可能归因于alg-2的下调作用，其导致ALG-2相关功能减弱，如细胞凋亡、囊泡出芽和蛋白质转运。
Autographa california multiple nucleopolyhedrovirus (AcMNPV)-encoded microRNAs (miRNAs) that regulate viral genes to achieve infection have been reported previously. Here, we report another AcMNPV encoded miRNA, AcMNPV-miR-4 (Ac-miR-4), which downregulated the host gene, apoptosis-linked gene (alg-2). This regulation was verified by dual-luciferase reporter assays. The effects of Ac-miR-4 on virus infection were assessed. The results showed that the production of infectious budded virions (BV) was decreased and the occlusion-derived virion (ODV) embedding into polyhedra was delayed when Sf9 cells were administered an overdose of Ac-miR-4. All these findings suggest that Ac-miR-4 prolongs cell lifespan and reduces virus virulence at a relatively early stage but increases ODV at a very late stage. This finding may be attributed to the downregulation effects of alg-2, which lead to weakened ALG-2 related functions, such as cell apoptosis, vesicle budding and protein transport.