Chemoenzymatic synthesis of α-dystroglycan core M1 O-mannose glycans.
Chemoenzymatic synthesis of α-dystroglycan core M1 O-mannose glycans.
复制标题
α-肌营养不良聚糖核心 M1 O-甘露糖聚糖的化学酶法合成
DOI：
10.1039/c5cc02913a
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发表时间：
2015-07-25
期刊：
影响因子：
--
通讯作者：
Cao H
中科院分区：
文献类型：
--
作者：
Zhang Y;Meng C;Jin L;Chen X;Wang F;Cao H
利用化学合成的双糖丝氨酸中间体，通过三步连续一锅多酶（OPME）糖基化，实现了面向多样性的化学酶合成α-三聚糖（α-DG）核心M1 o -甘露糖聚糖。该化学酶策略的高灵活性和高效率首次被证明用于合成三个更复杂的核心M1 o -甘露糖聚糖以及三个先前报道的核心M1结构。
The diversity-oriented chemoenzymatic synthesis of α-dystroglycan (α-DG) core M1 O-mannose glycans has been achieved via a three-step sequential one-pot multienzyme (OPME) glycosylation of a chemically prepared disaccharyl serine intermediate. The high flexibility and efficiency of this chemoenzymatic strategy was demonstrated for the synthesis of three more complex core M1 O-mannose glycans for the first time along with three previously reported core M1 structures.