TGF beta suppresses casein synthesis in mouse mammary explants and may play a role in controlling milk levels during pregnancy.
TGF beta suppresses casein synthesis in mouse mammary explants and may play a role in controlling milk levels during pregnancy.
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DOI：
10.1083/jcb.120.1.245
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发表时间：
1993-01
期刊：
影响因子：
--
通讯作者：
Daniel CW
中科院分区：
文献类型：
--
作者：
Robinson SD;Roberts AB;Daniel CW
从14-15天怀孕的小鼠乳腺外植体合成和分泌乳蛋白在培养响应胰岛素，氢化可的松，和催乳素。在这里，我们证明了转化生长因子β（TGF β）治疗抑制，在剂量依赖性和可逆的方式，外植体合成和分泌牛奶酪蛋白的能力。TGF β不影响外植体内酪蛋白mRNA的水平，但抑制酪蛋白的转录后合成。我们还发现，随着妊娠的进展，TGF β 2和TGF β 3在完整乳腺中的表达增加，而在哺乳期开始时，所有三种TGF β的表达减少。这些发现表明，内源性产生的TGF β可能会限制妊娠期间乳腺中产生的牛奶酪蛋白的积累。
Mammary explants from 14-15-d-pregnant mice synthesize and secrete milk proteins in culture in response to insulin, hydrocortisone, and prolactin. Here we demonstrate that transforming growth factor beta (TGF beta) treatment suppresses, in a dose dependent and reversible manner, the ability of explants to synthesize and secrete milk caseins. TGF beta does not affect the level of casein mRNA within explants but inhibits casein synthesis posttranscriptionally. We also show increased expression of TGF beta 2 and TGF beta 3 in intact mammary gland as pregnancy progresses, with reduced expression of all three TGF betas at the onset of lactation. These findings suggest that endogenously produced TGF beta may limit the accumulation of milk caseins that are produced in the mammary gland during pregnancy.