THE STRUCTURE OF HOBO TRANSPOSABLE ELEMENTS AND THEIR INSERTION SITES
THE STRUCTURE OF HOBO TRANSPOSABLE ELEMENTS AND THEIR INSERTION SITES
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DOI：
10.1002/j.1460-2075.1986.tb04690.x
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发表时间：
1986-12-01
期刊：
影响因子：
11.4
通讯作者：
BECKENDORF, SK
中科院分区：
文献类型：
--
作者：
STRECK, RD;MACGAFFEY, JE;BECKENDORF, SK
果蝇的流浪汉转座元件形成了一个由3.0 kb元件及其缺失衍生物组成的家族。他们的分布是一致的模型，3.0 kb的元件是功能完整的，但较小的流浪汉是有缺陷的，需要完整的元件在反式转座。一个3.0 kb的元件的序列，它有几个有趣的功能，包括一个1.9 kb的开放阅读框架下游潜在的TATA和CAT序列。11个独立插入位点的比较表明，在每种情况下，流浪汉元件已整合并复制序列NNNNNAC或CTTTNNNN。有证据表明，与这两个序列匹配的流浪汉内部的8个核苷酸序列已被用作第二个流浪汉元件的插入位点，作为产生内部缺失衍生物的第一步。流浪汉和真核生物转座因子P、Ac之间的结构相似性。1723，和Tam3，发现在广泛不同的宿主生物，表明他们都转座由一个共同的机制。
The hobo transposable elements of Drosophila form a family of 3.0-kb elements and their deletion derivatives. Their distribution is consistent with the model that 3.0-kb elements are functionally complete but that smaller hobos are defective and require complete elements in trans for transposition. The sequence of one 3.0-kb element is presented; it has several interesting features, including a 1.9-kb open reading frame downstream from potential TATA and CAT sequences. Comparison of 11 independent insertion sites shows that in every case the hobo element has integrated at and duplicated either the sequence NNNNNNAC or CTTTNNNN. There is evidence that an eight nucleotide sequence internal to hobo that matches both of these sequences has been used as an insertion site for a second hobo element, as the first step in the creation of an internal deletion derivative. Structural similarities between hobo and the eukaryotic transposable elements P, Ac. 1723, and Tam3, found in widely divergent host organisms, suggest that they all transpose by a common mechanism.