Duration of infection and antigen display have minimal influence on the kinetics of the CD4+ T cell response to Listeria monocytogenes infection
Duration of infection and antigen display have minimal influence on the kinetics of the CD4+ T cell response to Listeria monocytogenes infection
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DOI：
10.4049/jimmunol.173.9.5679
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发表时间：
2004-11-01
影响因子：
4.4
通讯作者：
Harty, JT
中科院分区：
文献类型：
--
作者：
Corbin, GA;Harty, JT
T细胞对感染的反应包括效应细胞的克隆扩增，然后收缩到记忆水平。以前认为感染的持续时间决定了T细胞应答的幅度和动力学。然而，最近的分析表明，Ag特异性CD 8（+）T细胞反应的扩张和收缩阶段之间的转换不受感染或Ag展示期间的实验操作的影响。我们研究了感染持续时间和Ag展示是否影响Ag特异性CD 4（+）T细胞对单核细胞增生李斯特菌（LM）感染的反应动力学。我们发现，早在感染后24小时，用抗生素治疗截断感染和Ag展示对CD 4（+）T细胞的扩增或收缩影响最小;然而，Ag特异性CD 4（+）和CD 8（+）T细胞应答的大小受到抗生素治疗时间的不同影响。在感染后24小时用抗生素治疗LM感染的小鼠，在感染后28天并没有阻止可检测的CD 4（+）和CD 8（+）记忆T细胞的产生，这些记忆T细胞的剧烈二次扩增，或对随后的LM攻击的保护。这些结果表明，在感染的最初几天内的事件刺激CD 4（+）和CD 8（+）T细胞应答，这些T细胞应答能够执行扩展和收缩的完整程序以形成功能性记忆，而与长期感染或Ag展示无关。
The T cell response to infection consists of clonal expansion of effector cells, followed by contraction to memory levels. It was previously thought that the duration of infection determines the magnitude and kinetics of the T cell response. However, recent analysis revealed that transition between the expansion and contraction phases of the Ag-specific CD8(+) T cell response is not affected by experimental manipulation in the duration of infection or Ag display. We studied whether the duration of infection and Ag display influenced the kinetics of the Ag-specific CD4(+) T cell response to Listeria monocytogenes (LM) infection. We found that truncating infection and Ag display with antibiotic treatment as early as 24 It postinfection had minimal impact on the expansion or contraction of CD4(+) T cells; however, the magnitudes of the Ag-specific CD4(+) and CD8(+) T cell responses were differentially affected by the timing of antibiotic treatment. Treatment of LM-infected mice with antibiotics at 24 h postinfection did not prevent generation of detectable CD4(+) and CD8(+) memory T cells at 28 days after infection, vigorous secondary expansion of these memory T cells, or protection against a subsequent LM challenge.. These results demonstrate that events within the first few days of infection stimulate CD4(+) and CD8(+) T cell responses that are capable of carrying out the full program of expansion and contraction to functional memory, independently of prolonged infection or Ag display.