Antioxidant activity of fucose-containing sulfated polysaccharides obtained from Fucus evanescens by different extraction methods
Antioxidant activity of fucose-containing sulfated polysaccharides obtained from Fucus evanescens by different extraction methods
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DOI：
10.1007/s10811-014-0293-7
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发表时间：
2015-02-01
影响因子：
3.3
通讯作者：
Zvyagintseva, Tatiana N.
中科院分区：
文献类型：
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作者：
Imbs, Tatiana I.;Skriptsova, Anna V.;Zvyagintseva, Tatiana N.
采用热酸提取法和氯化钙水溶液提取法从墨角藻（Fucus evanescens）中分离得到含岩藻糖的硫酸多糖（FCSPs），然后用阴离子交换层析法分离FCSPs。这些多糖级分的化学特性进行了测定。结果表明，用氯化钙水溶液提取提供了FCSP的纯化制剂。通过这种方法获得的粗多糖和其色谱纯化级分具有显着较低的海带多糖，糖醛酸，多酚类化合物的含量相比，通过热酸提取获得的FCSPs。所有多糖均表现出总的抗氧化能力和1，1-二苯基-2-吡肼基（DPPH）自由基清除能力，以及较低的2，2 '-连氮基-双3-乙基苯并噻唑啉-6-硫酸（ABTS）自由基清除能力。FCSPs中的多酚含量与其抗氧化活性呈显著正相关，这表明酚类物质在FCSPs的抗氧化能力中起着关键作用。
The fucose-containing sulfated polysaccharides (FCSPs) from Fucus evanescens were obtained via different procedures: by hot acidic extraction and with aqueous calcium chloride solution, followed by separation FCSPs using anion-exchanging chromatography. Chemical characteristics of these polysaccharide fractions were determined. It was shown that extraction with aqueous calcium chloride solution provides a purified preparation of the FCSPs. The crude polysaccharide and its chromatography purified fractions obtained by this method have significantly lower contents of laminaran, uronic acids, and polyphenols in comparison with FCSPs obtained by hot acid extraction. All polysaccharides exhibited total antioxidant capacity and 1,1-diphenyl-2-pycrilhydrazyl (DPPH) radical-scavenging activity and low 2,2'-azino-bis 3-ethyl benzothiazoline-6-sulfuric acid (ABTS) radical-scavenging activity. A strong positive correlation between polyphenol content in FCSPs and their antioxidant activity was found. This fact may indicate that phenols play a key role in the previously described antioxidant capacity of FCSPs.