NHERF family and NHE3 regulation
NHERF family and NHE3 regulation
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DOI：
10.1113/jphysiol.2005.090399
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发表时间：
2005-08-15
影响因子：
5.5
通讯作者：
Li, XH
中科院分区：
文献类型：
--
作者：
Donowitz, M;Cha, BY;Li, XH
肠和肾近端小管刷状边界（BB） Na+-H+交换器NHE3与NHERF （Na+-H+交换调节因子）家族成员结合。这是四种蛋白质（目前最常用的名称包括NHERF1， NHERF2， PDZK1和ippp），它们彼此相关，存在于BB内或靠近BB的位置，并以动态的方式在含NHE3复合物中支架一系列可变的蛋白质，这些蛋白质会因影响NHE3活性的信号转导变化而改变。这些蛋白质在NHE3调控中的具体作用以及它们彼此之间以及与许多其他底物的相互作用现在才被定义。在NHE3调控的一个例子中，通过cGMP的升高抑制NHERF家族成员的特异性被用来描述NHERF家族蛋白如何参与NHE3复合物的形成及其调控。在这种情况下，NHERF2直接与刷状边界的cGKII结合形成NHE3复合物，cGKII也通过其肉豆酰化与BB结合。
The intestinal and renal proximal tubule brush border (BB) Na+-H+ exchanger NHE3 binds to members of the NHERF (Na+-H+ exchanger regulatory factor) family. These are four proteins (current most used names include NHERF1, NHERF2, PDZK1 and IKEPP) which are related to each other, are present in locations in or close to the BB, and scaffold a variable series of proteins in NHE3-containing complexes in a dynamic manner that is altered by changes in signal transduction which affects NHE3 activity. The specific roles of these proteins in terms of NHE3 regulation as well as interactions with each other and with their many other substrates are only now being defined. Specificity for only one member of the NHERF family in one example of NHE3 regulation, inhibition by elevation in cGMP, is used to describe how NHERF family proteins are involved in NHE3 complex formation and its regulation. In this case, NHERF2 directly binds cGKII in the brush border to form an NHE3 complex, with cGKII also associating with the BB via its myristoylation.