Establishment of orthotopic mouse superficial bladder tumor model for studies on intravesical treatments
Establishment of orthotopic mouse superficial bladder tumor model for studies on intravesical treatments
复制标题
小鼠浅表膀胱肿瘤原位模型的建立用于膀胱内治疗研究
作者：
Y. Horiguchi;E. Kikuchi;C. Ozu;T. Nishiyama;M. Oyama;M. Horinaga;K. Yoshioka;M. Tachibana
已经开发了各种膀胱肿瘤的动物模型，用于临床前评价膀胱癌治疗的治疗方式。研究拟议的新型膀胱内治疗的治疗效果的理想模型要求至少在初始阶段将植入的肿瘤质量限制在膀胱的尿路上皮中。然而，先前报道的膀胱肿瘤模型不适合用于评价用于治疗浅表性膀胱癌的膀胱内疗法，因为肌肉浸润性肿瘤从实验开始就已经发展。这些模型过于激进，无法研究局部治疗效果。在目前的研究中，我们证明，小心地将MBT-2小鼠膀胱癌细胞滴注到同基因C3 H/HeJ小鼠的膀胱中可以建立浅表性膀胱肿瘤，发病率为100%。处理动物的程序和技术对于标准动物研究者来说很简单。维持MBT-2细胞的体外条件而不污染支原体和仔细选择C3 H小鼠的亚系似乎是稳定的肿瘤建立所必需的。这种膀胱肿瘤模型似乎很容易在不同机构的几个研究者中重现。原位膀胱肿瘤模型，这是局限于尿道，让我们评估各种膀胱内治疗策略。
Various animal models of bladder tumor have been developed for the preclinical evaluation of therapeutic modalities for the treatment of bladder cancers. The ideal model for the investigation of therapeutic effects of proposed novel intravesical treatments requires the mass of the implanted tumor to be confined to the urothelium of the bladder at least for the initial phase. However, previously reported bladder tumor models are not suitable for the evaluation of intravesical therapies for the treatment of superficial bladder cancer, since the muscle invasive tumors have developed from the beginnings of the experiments. These models are too aggressive to study local treatment effects. In the current study, we demonstrated that careful instillation of MBT-2 mouse bladder cancer cells into the bladder of a syngenic C3H/HeJ mouse could establish a superficial bladder tumor with an incidence of 100%. The procedure and technique for handling animals are simple for standard animal investigators. Maintenance of the in vitro conditions of MBT-2 cells without contamination of Mycoplasma and careful selection of the substrain of C3H mouse seem to be essential for stable tumor establishment. This bladder tumor model appeared to be easy to reproduce among several investigators in different institutions. The orthotopic bladder tumor model, which was confined to urothelium, lets us evaluate various intravesical treatment strategies.
使用细胞因子基因修饰的肿瘤疫苗对膀胱癌进行免疫治疗。
影响因子：
11.2
作者：
Saito,S;Bannerji,R;Gansbacher,B;Rosenthal,FM;Romanenko,P;Heston,WD;Fair,WR;Gilboa,E
通讯作者：
Gilboa,E
BCG 介导的抗肿瘤活性需要胸腺依赖性免疫反应。
DOI：
10.1016/s0022-5347(17)43909-7
发表时间：
1987
期刊：
The Journal of urology
影响因子：
--
作者：
Ratliff,TL;Gillen,D;Catalona,WJ
通讯作者：
Catalona,WJ
卡介苗治疗期间影响小鼠膀胱肿瘤植入重现性的技术因素。
影响因子：
11.2
作者：
Shapiro,A;Kelley,DR;Oakley,DM;Catalona,WJ;Ratliff,TL
通讯作者：
Ratliff,TL