Mucoid mutants of the biocontrol strain Pseudomonas fluorescens CHA0 show increased ability in biofilm formation on mycorrhizal and nonmycorrhizal carrot roots
Mucoid mutants of the biocontrol strain Pseudomonas fluorescens CHA0 show increased ability in biofilm formation on mycorrhizal and nonmycorrhizal carrot roots
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DOI：
10.1094/mpmi.2001.14.2.255
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发表时间：
2001-02-01
影响因子：
3.5
通讯作者：
Perotto, S
中科院分区：
文献类型：
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作者：
Bianciotto, V;Andreotti, S;Perotto, S
胞外多糖在细菌生物膜的形成中起着重要的作用。我们测试了两种突变菌株的生物膜形成能力，与野生型生物防治菌株荧光假单胞菌CHA0相比，它们的酸性胞外多糖产量增加。研究了细菌在丛枝菌根真菌Glomus intraradices的无菌根和菌根根以及根外菌丝体上的锚定作用。非黏液样野生型荧光假单胞菌CHA0几乎不粘附在所有表面，而两种黏液样菌株在根和真菌结构上形成致密的斑片状细菌层。促进植物生长的细菌黏附性能的提高可能导致混合接种体与根际间的相互作用更加稳定。
Extracellular polysaccharides play an important role in the formation of bacterial biofilms. We tested the biofilm-forming ability of two mutant strains with increased production of acidic extracellular polysaccharides compared with the wild-type biocontrol strain Pseudomonas fluorescens CHA0. The anchoring of bacteria to axenic nonmycorrhizal and mycorrhizal roots as well as on extraradical mycelium of the arbuscular mycorrhizal fungus Glomus intraradices was investigated. The nonmucoid wild-type strain P. fluorescens CHA0 adhered very little on all surfaces, whereas both mucoid strains formed a dense and patchy bacterial layer on the roots and fungal structures. Increased adhesive properties of plant-growth-promoting bacteria may lead to more stable interactions in mixed inocula and the rhizosphere.