Co-expression of Japanese encephalitis virus prM-E-NS1 antigen with granulocyte-macrophage colony-stimulating factor enhances humoral and anti-virus immunity after DNA vaccination
Co-expression of Japanese encephalitis virus prM-E-NS1 antigen with granulocyte-macrophage colony-stimulating factor enhances humoral and anti-virus immunity after DNA vaccination
复制标题
日本脑炎病毒prM-E-NS1抗原与粒细胞-巨噬细胞集落刺激因子的共表达增强DNA疫苗接种后的体液和抗病毒免疫力
DOI：
10.1016/j.imlet.2009.12.023
复制
发表时间：
2010-03-10
影响因子：
4.4
通讯作者：
An, Jing
中科院分区：
文献类型：
--
作者：
Gao, Na;Chen, Wei;An, Jing
日本脑炎病毒（JEV）是日本脑炎的一种病原体，粒细胞-巨噬细胞集落刺激因子（GM-CSF）因其抗原呈递而成为一种有吸引力的DNA疫苗佐剂。在本研究中，我们构建了携带JEV prM-E-NS1基因（含或不含GM-CSF基因）的DNA疫苗。用共表达GM-CSF和病毒prM-E-NS1的双胞质粒pCAG-JEGM免疫BALB/c小鼠，产生最高的IgG应答和足够的保护作用。然而，令我们惊讶的是，GM-CSF质粒与表达病毒prM-E-NS1的pCAG-JE质粒共接种导致抗体滴度低，存活率相对较低。此外，记忆抗体介导的保护在小鼠乙脑病毒攻击模型中起主导作用。根据激发后中和抗体滴度的增强和进一步的过继性转移实验。综上所述，这项研究应该鼓励进一步开发乙脑病毒DNA疫苗策略，并警告不要使用细胞因子作为佐剂。(C) 2010 Elsevier B.V.版权所有
Japanese encephalitis virus (JEV) is an agent of Japanese encephalitis, and granulocyte-macrophage colony-stimulating factor (GM-CSF) is an attractive DNA vaccine adjuvant for its antigen presentation. In the present study, we have constructed DNA vaccines that carried JEV prM-E-NS1 genes with or without the GM-CSF gene. Immunization with the bicistronic plasmid pCAG-JEGM that co-expresses GM-CSF and viral prM-E-NS1, resulted in the highest IgG response and sufficient protection against virus-challenged BALB/c mice. However, much to our surprise, co-inoculation of the GM-CSF plasmid with the pCAG-JE plasmid expressing viral prM-E-NS1 lead to a low antibody titer and a relatively low survival rate. Moreover, anamnestic antibody-mediated protection played a dominant role in the mice JEV challenge model. according to the enhancement of post-challenge neutralizing antibody titers and further adoptive transfer experiments. Taken together, this study should encourage further development of JEV DNA vaccine strategies and caution against the use of cytokines as an adjuvant. (C) 2010 Elsevier B.V. All rights reserved.