The development of functional B lymphocytes in conditional PU.1 knock-out mice
The development of functional B lymphocytes in conditional PU.1 knock-out mice
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DOI：
10.1182/blood-2005-01-0283
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发表时间：
2005-09-15
期刊：
影响因子：
20.3
通讯作者：
Nutt, SL
中科院分区：
文献类型：
--
作者：
Polli, M;Dakic, A;Nutt, SL
大量的研究已经确立了这样的观点，即含有转录因子PU.1的Ets结构域对B淋巴细胞的发育和功能是至关重要的。在这项研究中，我们使用了条件PU.1等位基因来测试这一概念。PU.1的完全缺失导致胎儿肝脏中B细胞和所有其他谱系阳性细胞的丢失以及E18.5和出生之间的死亡;然而，B谱系中PU.1的特异性缺失对B细胞发育没有影响。此外，B细胞中PU.1的缺失不会损害它们建立和维持免疫应答的能力。在B细胞受体（BCR）交联后，在体外观察到细胞凋亡水平增加;然而，这部分被白细胞介素-4（IL-4）挽救。这些发现表明，PU.1对于功能性B淋巴细胞在前B阶段之后的发育不是必需的。
An abundance of research has entrenched the view that the Ets domain containing transcription factor PU.1 is fundamental to the development and function of B lymphocytes. In this study, we have made use of a conditional PU.1 allele to test this notion. Complete deletion of PU.1 resulted in the loss of B cells and all other lineage-positive cells in the fetal liver and death between E18.5 and birth; however, specific deletion of PU.1 in the B lineage had no effect on B-cell development. Furthermore, deletion of PU.1 in B cells did not compromise their ability to establish and maintain an immune response. An increased level of apoptosis was observed in vitro upon B-cell receptor (BCR) cross-linking; however, this was partially rescued by interieukin-4 (IL-4). These findings suggest that PU.1 is not essential for the development of functional B lymphocytes beyond the pre-B stage.