Wilfoside K1N isolated from Cynanchum wilfordii inhibits angiogenesis and tumor cell invasion.
Wilfoside K1N isolated from Cynanchum wilfordii inhibits angiogenesis and tumor cell invasion.
复制标题
DOI：
10.3892/ijo.26.6.1533
复制
发表时间：
2005-06
影响因子：
5.2
通讯作者：
M. Kim;J. Baek;J. Park;B. Hwang;S. Kim;Jung Joon Lee;Kyu-Won Kim
中科院分区：
文献类型：
--
作者：
M. Kim;J. Baek;J. Park;B. Hwang;S. Kim;Jung Joon Lee;Kyu-Won Kim
Wilfoside K1 N是从萝摩科植物Cynanchum wilfordii中分离得到的一种聚氧甾烷糖苷。聚氧乙烯醚糖苷与细胞免疫和抗肿瘤活性相关，并增加许多抗癌药物对肿瘤细胞的细胞毒性，显示多药耐药活性。在本研究中，我们研究了wilfoside K1 N的抗血管生成和抗侵袭活性。在使用C57 BL/6小鼠的体内Matrigel plug测定中，wilfoside K1 N强烈抑制碱性成纤维细胞生长因子诱导的微血管形成。暴露于人脐静脉内皮细胞（HUVEC）的wilfoside K1 N抑制在体外管形成在不影响细胞活力的浓度。Wilfoside K1 N可显著抑制HUVEC和小牛肺动脉内皮细胞的增殖。另外，雷公藤多甙K1 N可降低HT 1080人纤维肉瘤细胞的体外侵袭能力，其抑制作用可能是通过下调基质金属蛋白酶9的活性和数量实现的。因此，我们目前的研究表明，wilfoside K1 N可能具有很强的抗血管生成和抗侵袭活性在体外和体内。
Wilfoside K1N is a polyoxypregnane glycoside isolated from Cynanchum wilfordii (Asclepiadaceae). Polyoxypregnane glycosides are associated with cellular immunity and anti-tumor activity, and increase the cytotoxicity of many anti-cancer drugs showing multidrug resistant activity on tumor cells. In the present study, we investigated the anti-angiogenic and anti-invasive activities of wilfoside K1N. In in vivo Matrigel plug assay using C57BL/6 mice, wilfoside K1N strongly inhibited basic fibroblast growth factor-induced microvessel formation. Exposure of wilfoside K1N to human umbilical vein endothelial cells (HUVEC) suppressed in vitro tube formation at a concentration not affecting cell viability. Moreover, wilfoside K1N significantly reduced the proliferation of HUVEC and calf pulmonary artery endothelial cells. In addition, wilfoside K1N decreased in vitro invasion of HT1080 human fibrosarcoma cells, and the inhibition might be through down-regulation of activity as well as quantity of matrix metalloproteinase-9. Therefore, our present study suggests that wilfoside K1N may have a potential to have strong anti-angiogenic and anti-invasive activities both in vitro and in vivo.