Optimization of spectral light quality for growth and product formation in different microalgae using a continuous photobioreactor
Optimization of spectral light quality for growth and product formation in different microalgae using a continuous photobioreactor
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DOI：
10.1016/j.algal.2016.01.011
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发表时间：
2016-03-01
影响因子：
5.1
通讯作者：
Huebner, Holger
中科院分区：
文献类型：
--
作者：
Baer, Sascha;Heining, Martin;Huebner, Holger
到目前为止，微藻培养的许多参数还没有完全优化。尽管光是一个重要的过程参数，但关于它的光谱组成的研究很少。与其他已发表的数据(主要是单色或双色照明)不同，本研究的目的是通过保持总的光强恒定，研究广泛的不同光质对细胞干重生产率以及藻胆蛋白藻红蛋白或藻蓝蛋白生产率的影响。不是分批培养，而是在封闭的、热灭菌的光生物反应器中进行连续培养。在这幅作品中，总共展示了37种轻质条件。以莱茵衣藻为例，当α(红)：α(绿)：α(蓝)为80：10：10时，生物产量最高，为252mg.L-1.d(-1)。在纯红光(304mg.L-1.d(-1))下，硫藻的生物量生产力达到最大值，而当α(红)：α(绿)：α(蓝)为60：0：40(4.99mg.L-1.d(-1))时，藻蓝蛋白产量达到最高。在α(红)：α(绿)：α(蓝)=40：40：20时，紫球藻的生物量和藻红蛋白产量最高，分别为312mg.L-1.d(-1)和16.93mg.L-1.d(-1)。收集的数据清楚地表明了为每个微藻菌株和所需的感兴趣的产品单独研究正确的光混合的意义。(C)2016爱思唯尔B.V.保留所有权利。
Many parameters for microalgae cultivation are not fully optimized so far. Even though light is one important process parameter, little research is done regarding its spectral composition. In contrast to other published data, mostly with monochromatic or dichromatic illumination, the objective of this study was to investigate the influence of a broad range of different light qualities to the productivity of cell dry weight as well as the productivity of their phycobiliproteins phycoerythrin or phycocyanin, by keeping the overall light intensity constant. Instead of cultivating in batch mode, cultivations were done in continuous mode in closed, thermal sterilizable photobioreactors. A total of 37 light quality conditions are shown in this work. In case of Chlamydomonas reinhardtii a composition of alpha(red): alpha(green): alpha(blue) of 80:10:10 resulted in the highest biomass productivity, with a value of 252 mg.L-1.d(-1). A maximum biomass productivity is achieved for Galdieria sulphuraria at pure red light (304 mg.L-1.d(-1)), whereas the highest phycocyanin productivity is reached at a ratio of alpha(red): alpha(green): alpha(blue) of 60:0:40 (4.99 mg.L-1.d(-1)). The highest biomass and phycoerythrin productivity was achieved for Porphyridium purpureum at alpha(red) : alpha(green) : alpha(blue) of 40:40:20, with values of 312 mg.L-1.d(-1) and 16.93 mg.L-1.d(-1), respectively. The collected data showclearly the significance to investigate the right mixture of light for every microalgal strain and the desired product of interest individually. (C) 2016 Elsevier B.V. All rights reserved.