THERMAL ENHANCEMENT OF DNA DAMAGE BY AN ALKYLATING AGENT IN HUMAN CELLS
THERMAL ENHANCEMENT OF DNA DAMAGE BY AN ALKYLATING AGENT IN HUMAN CELLS
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DOI：
10.1016/0006-291x(73)91046-2
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发表时间：
1973-01-01
影响因子：
3.1
通讯作者：
REGAN, JD
中科院分区：
文献类型：
--
作者：
BRONK, BV;WILKINS, RJ;REGAN, JD
在用甲磺酸甲酯处理期间和处理后，在不同温度下孵育人皮肤细胞，然后通过碱性蔗糖沉降法估计引入细胞DNA的单链断裂数。温度升高到37°以上，会大大增强甲基磺酸甲酯对DNA的损伤。DNA修复中的一个重要步骤失活，通过观察到DNA断裂的重新连接在临界温度（约41.5°）以上停止。DNA损伤程度随温度升高而增加，在42 ℃以上尤为明显。中国仓鼠细胞也获得了类似的结果。这些结果与细胞活力的相关性进行了讨论。
Human skin cells were incubated at various temperatures during and after treatment with methyl methanesulfonate and the number of single-strand breaks introduced into the cellular DNA then estimated by alkaline sucrose sedimentation. Elevation of temperature above 37° greatly enhanced damage to the DNA caused by methyl methanesulfonate. Inactivation of an essential step in the repair of DNA was indicated by the observation that rejoining of breaks in the DNA was halted above a critical temperature (about 41.5°). Enhancement of damage to DNA increased with temperature, especially above 42°. Similar results were obtained for Chinese hamster cells. A correlation of these results with cell viability is discussed.