Factors leading to tripronucleate eggs during human in-vitro fertilization.
Factors leading to tripronucleate eggs during human in-vitro fertilization.
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人类体外受精过程中导致三核卵的因素。
DOI：
10.1093/oxfordjournals.humrep.a136354
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发表时间：
1986
影响因子：
6.1
通讯作者：
F. Leroy
中科院分区：
文献类型：
--
作者：
Y. Englert;F. Puissant;M. Camus;M. Degueldre;F. Leroy
491个卵母细胞从142个连续参加体外受精的患者中收集。授精后14 ~ 18 h对原核进行系统观察，发现391个受精卵中有27个为三原核卵（6.9%），与文献报道的比率一致。分析了以下参数与这些卵子发生率的关系：不孕症的病因、卵泡对激素刺激的反应、排卵刺激的类型、精子计数和活力以及受精率。只有受精率和浓度的活动精子在授精培养基中被发现与三原核卵，确认该条件主要是由于多精受精。与动物的数据比较，并提出了旨在防止多精受精的措施。
Four-hundred-and-ninety-one oocytes were collected from 142 successive patients attending for in-vitro fertilization. The systematic observation of pronuclei between 14 and 18 h after insemination revealed 27 cases of tripronucleate eggs among 391 fertilized eggs (6.9%), which corresponds to rates generally reported in the literature. The following parameters were analysed in relation to the incidence of these eggs: aetiology of infertility, follicular response to hormonal stimulation, type of ovulatory stimulus, sperm count and motility and the incidence of fertilization. Only fertilization rates and concentration of motile spermatozoa in the insemination medium were found to be correlated with tripronucleate eggs, confirming that the condition is predominantly due to polyspermy. Comparisons with data from animals, and measures aimed at preventing polyspermy are suggested.