Behavioral analysis of the huntingtin-associated protein 1 ortholog trak-1 in Caenorhabditis elegans.
Behavioral analysis of the huntingtin-associated protein 1 ortholog trak-1 in Caenorhabditis elegans.
复制标题
DOI：
10.1002/jnr.23756
复制
发表时间：
2016-09
影响因子：
4.2
通讯作者：
Gutekunst CA
中科院分区：
文献类型：
--
作者：
Norflus F;Bu J;Guyton E;Gutekunst CA
亨廷顿蛋白相关蛋白1（HAP 1）的确切作用尚不清楚，但研究表明它对早期发育和生存很重要。本文报道了秀丽隐杆线虫（C. habditiselegans，C. elegans）的直系同源物（T27A3.1，也称为trak-1），并且在神经元的子集中表达。检测了T27A3.1的三个敲除系的潜在行为功能。基于T27A3.1在小鼠中的作用，我们推测T27A3.1可能参与卵孵化和早期生长、机械感觉、化学感觉、渗透压敏感性和突触传递。我们的研究表明，敲除蠕虫与野生型蠕虫仅在突触传递试验中有显著差异，该试验通过添加乙酰胆碱酯酶抑制剂涕灭威来测量。通过测量瘫痪的蠕虫数量来确定功能的变化。然而，当测试T27A3.1蠕虫的卵孵化和早期生长、机械感觉、化学感觉和对渗透压的敏感性时，敲除蠕虫和野生型蠕虫之间没有显著差异。亨廷顿蛋白相关蛋白的秀丽隐杆线虫直系同源物HAP 1已被鉴定为trak-1，并在神经元亚群中表达。对敲除蠕虫进行了行为测试，结果表明，与野生型蠕虫相比，它们在突触传递方面存在缺陷。
The precise role of Huntingtin Associated Protein 1 (HAP1) is not known but studies have shown that it is important for early development and survival. A Caenorhabditis elegans (C. elegans) ortholog of HAP1 (T27A3.1 also called trak-1) has been found and is expressed in a subset of neurons. Potential behavioral functions of three knockout lines of T27A3.1 were examined. Based on its suspected role in mice, we hypothesized that T27A3.1 might be involved in egg hatching and early growth, mechanosensation, chemosensation, sensitivity to osmolarity and synaptic transmission. Our studies show that the knockout worms are significantly different from the wildtype worms in only the synaptic transmission test which was measured by adding aldicarb, an acetylcholinesterase inhibitor. The change in function was determined by measuring the number of worms paralyzed. However, when the T27A3.1 worms were tested for egg hatching and early growth, mechanosensation, chemosensation and sensitivity to osmolarity, there were no significant differences between the knockout and wildtype worms. A Caenorhabditis elegans ortholog of Huntingtin Associated Protein, HAP1 has been identified as trak-1 and is expressed in a subset of neurons. Behavioral tests were performed on the knockout worms and reveal that they have defects in synaptic transmission compared to wildtype worms.