THE MATERNAL GENE SKN-1 ENCODES A PROTEIN THAT IS DISTRIBUTED UNEQUALLY IN EARLY C-ELEGANS EMBRYOS
THE MATERNAL GENE SKN-1 ENCODES A PROTEIN THAT IS DISTRIBUTED UNEQUALLY IN EARLY C-ELEGANS EMBRYOS
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DOI：
10.1016/0092-8674(93)80046-h
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发表时间：
1993-08-13
期刊：
影响因子：
64.5
通讯作者：
PRIESS, JR
中科院分区：
文献类型：
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作者：
BOWERMAN, B;DRAPER, BW;PRIESS, JR
线虫早期胚胎卵裂球的自主或细胞固有发育特性被认为是由母体提供的决定因素的作用引起的。线虫胚胎第一次卵裂后，只有后卵裂球 P1 具有产生咽细胞的细胞固有能力。 P1 产生咽细胞需要母体基因 skn-1 的产物。我们在此表明 Skn-1 蛋白位于核内，并且 P1 似乎比其姐妹 AB 卵裂球积累了明显更高水平的 Skn-1 蛋白。我们研究了基因 mex-1、par-1 和ie-1 发生母体效应突变的母亲胚胎中 Skn-1 蛋白的分布。这些结果表明，mex-1(+)和par-1(+)活性是Skn-1蛋白不均匀分布所必需的，并且pie-1(+)活性可能起到调节后卵裂球P1后代中Skn-1蛋白活性的作用。
The autonomous or cell-intrinsic developmental properties of early embryonic blastomeres in nematodes are thought to result from the action of maternally provided determinants. After the first cleavage of the C. elegans embryo, only the posterior blastomere, P1, has a cell-intrinsic ability to produce pharyngeal cells. The product of the maternal gene skn-1 is required for P1 to produce pharyngeal cells. We show here that the Skn-1 protein is nuclear localized and that P1 appears to accumulate markedly higher levels of Skn-1 protein than its sister, the AB blastomere. We have examined the distribution of Skn-1 protein in embryos from mothers with maternal-effect mutations in the genes mex-1, par-1, and pie-1. These results suggest that mex-1(+) and par-1(+) activities are required for the unequal distribution of the Skn-1 protein and that pie-1(+) activity may function to regulate the activity of Skn-1 protein in the descendants of the posterior blastomere P1.