Validation of a combined Fast blue BB and 4-Aminophenol colorimetric test for indication of Hemp-type and Marijuana-type cannabis
Validation of a combined Fast blue BB and 4-Aminophenol colorimetric test for indication of Hemp-type and Marijuana-type cannabis
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DOI：
10.1016/j.forc.2022.100448
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发表时间：
2022-09-20
影响因子：
2.7
通讯作者：
Almirall, Jose
中科院分区：
文献类型：
--
作者：
Acosta, Alexander;Li, Li;Almirall, Jose
用于指示大麻型和大麻型大麻的两种有效的推定测试是固蓝BB（FBBB）和4-氨基苯酚（4-AP）比色测试。我们报告的小型化的4-AP比色反应的基板上使用的组合与先前报道的FBBB测试。两种测试都使用90%的正确分类）将富含THC的大麻（THC：CBD > 2）与大麻单独分类，但当两种测试组合使用时，特异性略高（大于或等于95%正确分类）。THC：CBD低于2的Maritime样本被认为是SIMCA模型的离群值。然而，当去除这些样品时，SIMCA模型的灵敏度和特异性达到95%以上。这些观察和统计结果表明，FBBB和4-AP可以单独或组合使用，以可靠地指示大麻和大麻时，THC：CBD是高于2。
Two effective presumptive tests used to indicate hemp-type and marijuana-type cannabis are the Fast Blue BB (FBBB) and 4-Aminophenol (4-AP) colorimetric tests. We report the miniaturization of a 4-AP colorimetric re-action on a substrate to be used in combination with the previously reported FBBB test. Both tests use 90 % correct classification) at classifying THC-rich marijuana (THC:CBD > 2) from hemp individually but have a slightly higher specificity when both tests are used in combination (greater than or equal to 95 % correct classification). Marijuana samples with a THC:CBD below two were considered outliers for the SIMCA models. However, sensitivity and specificity above 95 % were achieved with the SIMCA models when these samples were removed. These observations and statistical results suggest that FBBB and 4-AP may be used either individually or in combination to reliably indicate hemp and marijuana when the THC:CBD is above two.