A new method for determining the minimum inhibitory concentration of essential oils
A new method for determining the minimum inhibitory concentration of essential oils
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DOI：
10.1046/j.1365-2672.1998.00379.x
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发表时间：
1998-04-01
影响因子：
4
通讯作者：
Markham, JL
中科院分区：
文献类型：
--
作者：
Mann, CM;Markham, JL
建立了一种测定油基化合物最低抑菌浓度（MIC）的新方法。氧化还原染料刃天青用于测定互叶白千层（茶树）精油样品对一系列革兰氏阳性和阴性细菌的MIC。使用0.15%（w/v）琼脂作为稳定剂克服了油和测试细菌之间充分接触的问题，并且避免了使用化学乳化剂的需要。还开发了该测定的快速版本，用作筛选方法。微量滴定板的目视和光度阅读比较表明，无需仪器即可评估结果;此外，如果使用快速测定形式，则无需严格的无菌操作。通过微孔板计数确认刃天青方法的准确度，并将MIC值与使用琼脂稀释试验获得的结果进行比较。刃天青法测得的MIC结果略低于琼脂稀释法测得的MIC结果。
A new microdilution method has been developed for determining the minimum inhibitory concentration (MIC) of oil-based compounds. The redox dye resazurin was used to determine the MIC of a sample of the essential oil of Melaleuca alternifolia (tea tree) for a range of Gram-positive and -negative bacteria. Use of 0.15% (w/v) agar as a stabilizer overcame the problem of adequate contact between the oil and the test bacteria and obviated the need to employ a chemical emulsifier. A rapid version of the assay was also developed for use as a screening method. A comparison of visual and photometric reading of the microtitre plates showed that results could be assessed without instrumentation; moreover, if the rapid assay format was used, rigorous asepsis was not necessary. Accuracy of the resazurin method was confirmed by plate counting from microwells and MIC values were compared with results obtained using an agar dilution assay. The MIC results obtained by the resazurin method were slightly lower than those obtained by agar dilution.