Expression of a harpin-encoding gene in rice confers durable nonspecific resistance to Magnaporthe grisea.
Expression of a harpin-encoding gene in rice confers durable nonspecific resistance to Magnaporthe grisea.
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DOI：
10.1111/j.1467-7652.2007.00304.x
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发表时间：
2007-11
影响因子：
13.8
通讯作者：
Min Shao;Jinsheng Wang;R. Dean;Yongjun Lin;Xuewen Gao;Shuijin Hu
中科院分区：
文献类型：
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作者：
Min Shao;Jinsheng Wang;R. Dean;Yongjun Lin;Xuewen Gao;Shuijin Hu
对植物病原体进行持久的非特异性抗性是植物育种的最终目标之一。然而，由于病原体种群的快速变化和病原体感染机制的多样性，实现这一目标的大多数尝试都失败了。在这项研究中，我们证明源自米黄单胞菌 pv 的 harpin 编码基因 (hrf1) 的表达。 oryzae，赋予水稻对稻瘟病菌 Magnaporthe grisea 的非特异性抗性。转基因植物及其 T1-T7 后代对中国长江沿岸水稻种植区的所有主要稻瘟病菌品种均具有高度抗性。在抗性转基因植物中，防御相关基因的表达被激活，并且植物叶子上黑化附着胞的形成受到抑制，黑化附着胞对于叶面感染至关重要。这些结果表明，harpins 可能为其他作物产生广谱抗病性提供新的机会。
Engineering durable nonspecific resistance to phytopathogens is one of the ultimate goals of plant breeding. However, most attempts to reach this goal fail as a result of rapid changes in pathogen populations and the sheer diversity of pathogen infection mechanisms. In this study, we show that the expression of a harpin-encoding gene (hrf1), derived from Xanthomonas oryzae pv. oryzae, confers nonspecific resistance in rice to the blast fungus Magnaporthe grisea. Transgenic plants and their T1-T7 progenies were highly resistant to all major M. grisea races in rice-growing areas along the Yangtze River, China. The expression of defence-related genes was activated in resistant transgenic plants, and the formation of melanized appressoria, which is essential for foliar infection, was inhibited on plant leaves. These results suggest that harpins may offer new opportunities for generating broad-spectrum disease resistance in other crops.