Immunohisto- and cytochemical examinations in labial salivary glands from Sjögren's syndrome patients with sensory neuropathy
Immunohisto- and cytochemical examinations in labial salivary glands from Sjögren's syndrome patients with sensory neuropathy
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干燥综合征感觉神经病患者唇唾液腺的免疫组织和细胞化学检查
DOI：
10.1055/s-2004-827110
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
E. Fehér
中科院分区：
文献类型：
--
作者：
B. Batbayar;G. Nagy;T. Zelles;E. Fehér
方法：采用免疫组织化学和细胞化学方法对正常对照组和SS腺体内的IR神经纤维进行检测，并与正常对照组进行比较。结果：正常对照组和SS腺体内均可见不同数量和定位的IR神经纤维。其密度在SS中有不同程度的变化。P物质(SP)、神经肽Y(NPY)、甘丙素(GAL)IR神经末梢减少，血管活性肠多肽(VIP)和酪氨酸β羟基酶(TH)IR神经纤维增加(P<；0.05)。SS中未检测到聚(ADP-核糖)聚合酶的激活。对照材料中未见IR免疫活性细胞，但在SS中大量表达SP和NPY。结论：这些神经肽可能在感觉神经病中起作用，它们可能激活了伤害性和交感通路。一些神经肽(SP、NPY)在免疫系统中是内源性的，在某些情况下如炎症和慢性自身免疫性疾病如SS等产生，因此它们可能参与神经免疫调节，促进萎缩、细胞凋亡和坏死。
Methods: Immunohisto-and cytochemical methods were used for the detection of immunoreactive (IR) elements and the data were compared with the healthy controls.Results: All of the investigated IR nerve fibres were found in different quantity and localization in both of control and SS glands. The density of them was changed variously in SS. The number of the substance P (SP), neuropeptide Y (NPY)(p< 0.05), galanin (GAL) IR nerve terminals was decreased, however the number of vasoactive intestinal polypeptide (VIP) and tyrosine β-hydroxylase (TH) IR nerve fibres (p< 0.05) was increased compared to the control. No Poly (ADP-ribose) polymerase activation was detected in SS. There were no IR immunocompetent cells in the control materials, however, a large number of them showed IR for SP and NPY in the SS.Conclusion: These neuropeptides might have a role in the sensory neuropathy; they might activate nociceptive and sympathetic pathways. Some neuropeptides (SP, NPY) are endogenous in the immunosystem and produced in certain conditions eg inflammation and chronic autoimmune disorders such as SS, so they might participate in the neuro-immunomodulation and contribute to the atrophy, apoptosis and necrosis.