Characterization of dermacan, a novel zebrafish lectican gene, expressed in dermal bones
Characterization of dermacan, a novel zebrafish lectican gene, expressed in dermal bones
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DOI：
10.1016/j.mod.2004.01.007
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发表时间：
2004-03-01
影响因子：
2.6
通讯作者：
Ninomiya, Y
中科院分区：
文献类型：
--
作者：
Kang, JS;Oohashi, T;Ninomiya, Y
我们在此报道了编码斑马鱼真皮聚糖的cDNA的分离和鉴定，这是透明质蛋白（HA）结合蛋白聚糖的新成员，其在斑马鱼真皮骨中的特征表达被命名。推导出的蛋白质序列具有典型的电蛋白模块元素。覆盖c端球状结构域的序列比较表明，dermacan与斑马鱼版本具有高度的同源性，但与任何其他已鉴定的lecticans不同。基因组DNA分析表明，dermacan和versican由斑马鱼基因组中不同的基因编码。dermacan的表达始于受精后16岁时的硬核层和头侧近轴中胚层。在咽部时期，在硬膜、尾鳍芽、咽弓原始区和耳囊中检测到皮蛋白转录本。在颅面骨发育过程中，真皮蛋白的表达主要见于骨周和牙髓。这些区域属于颅面真皮骨。相比之下，在颅面软骨骨元素中观察到聚集蛋白表达。在注射了真皮-morpholino的胚胎中，真皮骨（如表皮、牙髓和鳃骨线）以及躯干中轴骨骼的骨化程度降低。我们得出结论，derinacan是一种新的反射基因，斑马鱼的反射基因有遗传分化。此外，我们的数据表明dermacan参与斑马鱼真皮骨发育。2004爱思唯尔爱尔兰有限公司版权所有。
We report here the isolation and characterization of a cDNA encoding zebrafish dermacan, a novel member of hyalurcman (HA)-binding proteoglycans, which was termed after its characteristic expression in the zebrafish dermal bones. The deduced protein sequence shares the typical modular elements of lecticans. Sequence comparison covering the C-terminal globular domain demonstrated that dermacan shows high homology with zebrafish versican but is distinct from any other identified lecticans. Genomic DNA analysis demonstrated that dermacan and versican were encoded by distinct genes in the zebrafish genome. The expression of dermacan is initiated in the sclerotome and cephalic paraxial mesoderm at 16 It postfertilization. During the pharyngular period, dermacan transcripts were detected in the sclerotome, tail fin bud, pharyngular arch primordial region, and otic vesicle. In the development of craniofacial bones, dermacan expression was detected typically in the opercle and dentary. These regions belong to the craniofacial dermal bones. aggrecan expression, in contrast, was observed in the elements of craniofacial cartilage bones. In the dermacan-morpholino-injected embryos, dermal bones, e.g. opercle, dentary, and branchiostegal rays, as well as axial skeleton in the trunk, showed decreased ossification. We conclude that derinacan is a novel lectican gene, and that zebrafish lectican genes have genetically diverged. In addition, our data suggest the involvement of dermacan in zebrafish dermal bone development. (C) 2004 Elsevier Ireland Ltd. All rights reserved.