Possible participation of acidic pH in bone resorption in middle ear cholesteatoma.
Possible participation of acidic pH in bone resorption in middle ear cholesteatoma.
复制标题
酸性pH可能参与中耳胆脂瘤的骨吸收。
DOI：
10.1002/lary.23883
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Doi Y.
中科院分区：
文献类型：
--
作者：
Nguyen KH;Suzuki H;Ohbuchi T;Wakasugi T;Koizumi H;Hashida K;Baba R;Morimoto H;Doi Y.
目的/假设中耳炎性骨吸收的病理机制尚不清楚。我们研究了中耳胆脂瘤组织的pH值和胆脂瘤上皮细胞的渗透性，试图阐明骨吸收的机制，在这种diseases.Study Designemarkatorialstudy. Methods胆脂瘤组织收集从原发性获得性中耳胆脂瘤患者。使用pH计测量中耳炎瘤的角蛋白碎片的pH。在激光共聚焦显微镜和透射电子显微镜下观察中耳乳突瘤上皮。用荧光免疫组织化学和定量逆转录-聚合酶链反应（RT-PCR）方法检测了角蛋白丝聚蛋白在中耳炎组织中的表达。中耳乳突瘤上皮基底层pH值明显低于胃窦粘膜。透射电镜下可见镧在正常皮肤的颗粒层、棘层和基底层的细胞间隙中有明显的渗透。丝聚蛋白mRNA的表达显着降低在中耳炎组织比在正常skin.ConclusionsThese结果表明，酸渗漏通过中耳炎上皮可能参与了下面的骨结构的吸收。中耳炎上皮的渗透性增加可以通过聚丝蛋白表达的减少来解释。证据水平N/A.Laryngoscope，124：245-250，2014
Objectives/HypothesisThe etiopathology of bone resorption in cholesteatoma is unclear. We studied pH in middle ear cholesteatoma tissue and the permeability of the cholesteatoma epithelium in an attempt to elucidate the mechanism of bone resorption in this disease.Study DesignLaboratorial study.MethodsCholesteatoma tissue was collected from patients with primary acquired middle ear cholesteatoma. The pH of the keratin debris of cholesteatoma was measured using a pH meter. The cholesteatoma epithelium was examined under a confocal laser scanning microscope, and under a transmission electron microscope. Expression of filaggrin in the cholesteatoma tissue was explored by fluorescence immunohistochemistry and by quantitative reverse transcription‐polymerase chain reaction.ResultsThe pH of the keratin debris of cholesteatoma was acidic. The pH of the basal layer of the cholesteatoma epithelium was significantly lower than that of the antrum mucosa. Transmission electron microscope showed distinct penetration of lanthanum in the intercellular space of the basal, spinous, and granular layers of the cholesteatoma epithelium, but only a small amount of lanthanum in the granular layer in the normal skin. The expression of filaggrin mRNA was significantly lower in the cholesteatoma tissue than in the normal skin.ConclusionsThese results indicate that acid leakage through the cholesteatoma epithelium probably participates in the resorption of the underlying bone structure. The increased permeability of the cholesteatoma epithelium may be explained by a decrease in filaggrin expression.Level of EvidenceN/A.Laryngoscope, 124:245–250, 2014